Detailed information NTG0368754

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: GXM24_RS04740
• Genome accession: NZ_CP048439
• Coordinates: 995685..996677 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli strain NBRC 3301
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 990685..1001677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GXM24_RS04710 (GXM24_04710)	ispD	990746..991456 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
GXM24_RS04715 (GXM24_04715)	ispF	991456..991935 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
GXM24_RS04720 (GXM24_04720)	truD	991932..992981 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
GXM24_RS04725 (GXM24_04725)	surE	992962..993723 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
GXM24_RS04730 (GXM24_04730)	pcm	993717..994343 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
GXM24_RS04735 (GXM24_04735)	nlpD	994528..995622 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
GXM24_RS04740 (GXM24_04740)	rpoS	995685..996677 (+)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
GXM24_RS04745 (GXM24_04745)	ygbN	996771..998135 (-)	1365	WP_000104441.1	GntP family transporter	-
GXM24_RS04750 (GXM24_04750)	ygbM	998224..999000 (-)	777	WP_001136934.1	HPr family phosphocarrier protein	-
GXM24_RS04755 (GXM24_04755)	ygbL	999005..999643 (-)	639	WP_001278994.1	aldolase	-
GXM24_RS04760 (GXM24_04760)	otnK	999735..1000901 (-)	1167	WP_001393459.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=368754 GXM24_RS04740 WP_000081588.1 995685..996677(+) (rpoS) [Escherichia coli strain NBRC 3301]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=368754 GXM24_RS04740 WP_000081588.1 995685..996677(+) (rpoS) [Escherichia coli strain NBRC 3301]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715