Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPX93_RS01340 Genome accession   NZ_CP046749
Coordinates   278129..279856 (+) Length   575 a.a.
NCBI ID   WP_001122151.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1118     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273129..284856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX93_RS01315 gspM 273487..274137 (+) 651 WP_123162349.1 type II secretion system protein GspM -
  GPX93_RS01320 - 274130..274447 (+) 318 WP_089072027.1 MSHA biogenesis protein MshK -
  GPX93_RS01325 mshL 274462..276141 (+) 1680 WP_123162350.1 pilus (MSHA type) biogenesis protein MshL -
  GPX93_RS01330 - 276146..276991 (+) 846 WP_000281356.1 ExeA family protein -
  GPX93_RS01335 - 276988..278139 (+) 1152 WP_184482577.1 tetratricopeptide repeat protein -
  GPX93_RS01340 pilB/pilB1 278129..279856 (+) 1728 WP_001122151.1 GspE/PulE family protein Machinery gene
  GPX93_RS01345 - 279865..281088 (+) 1224 WP_123162352.1 type II secretion system F family protein -
  GPX93_RS01350 - 281091..281543 (+) 453 WP_000689652.1 hypothetical protein -
  GPX93_RS01355 - 281624..282178 (+) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPX93_RS01360 - 282213..282650 (+) 438 WP_000828595.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPX93_RS01365 - 282712..283224 (+) 513 WP_050555758.1 type II secretion system protein -
  GPX93_RS01370 - 283214..283783 (+) 570 WP_001144517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPX93_RS01375 - 283783..284553 (+) 771 WP_071186802.1 type II secretion system protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63881.35 Da        Isoelectric Point: 6.1324

>NTDB_id=354819 GPX93_RS01340 WP_001122151.1 278129..279856(+) (pilB/pilB1) [Vibrio cholerae strain 2015V-1118]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNHLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=354819 GPX93_RS01340 WP_001122151.1 278129..279856(+) (pilB/pilB1) [Vibrio cholerae strain 2015V-1118]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGGGCGCTGGTGATAGGCCGCAGTGGCGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCACTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTACTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCAGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilB Legionella pneumophila strain ERS1305867

39.245

92.174

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362