Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   GFX43_RS04975 Genome accession   NZ_CP046448
Coordinates   974223..976547 (+) Length   774 a.a.
NCBI ID   WP_015483691.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZD01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 969223..981547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFX43_RS04955 (GFX43_004955) bdbD 971214..971882 (+) 669 WP_003228414.1 disulfide bond formation protein BdbD -
  GFX43_RS04960 (GFX43_004960) bdbC 971887..972303 (+) 417 WP_015384637.1 disulfide oxidoreductase -
  GFX43_RS04965 (GFX43_004965) yvgT 972490..973101 (+) 612 WP_003228419.1 trimeric intracellular cation channel family protein -
  GFX43_RS04970 (GFX43_004970) - 973211..973645 (-) 435 WP_014477979.1 hypothetical protein -
  GFX43_RS04975 (GFX43_004975) helD 974223..976547 (+) 2325 WP_015483691.1 DNA helicase IV Machinery gene
  GFX43_RS04980 (GFX43_004980) cysJ 976718..978535 (+) 1818 WP_015483690.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  GFX43_RS04985 (GFX43_004985) cysI 978561..980276 (+) 1716 WP_015384634.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89867.42 Da        Isoelectric Point: 6.6088

>NTDB_id=352337 GFX43_RS04975 WP_015483691.1 974223..976547(+) (helD) [Bacillus subtilis strain ZD01]
MNQQDKEWKEEQSRIDEVLQELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREH
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEEQAERIYIGLASCLDEKEEHYLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKILIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFINEYVTRLSSDGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
SQLYLQLFSGWGGKFQHEKMAAIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTIHGDQRMDACFEDEPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRTGEMPLVF
KTEGHESLCQKLAQEIGRLKKKGHETIAVICKAAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGAASPFVTAVPSRLYQNAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=352337 GFX43_RS04975 WP_015483691.1 974223..976547(+) (helD) [Bacillus subtilis strain ZD01]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGCAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAC
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTAAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGAGCAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTATCTGA
TTTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGTAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGTGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGTGGCGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCCAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGACGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTCGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACCTGGACGTGTACAAAAAACTGCAAGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAGCAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
TCACAGCTGTATTTGCAATTGTTTTCTGGCTGGGGAGGGAAATTTCAGCATGAGAAGATGGCCGCGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGCATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATACACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGAGCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAACCGGGGAAATGCCGCTCGTTTTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGACGGCTCAAGAAAAAAGGGCACGAAACAAT
AGCCGTCATCTGCAAGGCTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTCCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCGGTATTTTATACAGGTGCAGCCAGTCCGTTTGTAACAGCTGTGCCGTCTCGCTTATATCAGAATGCTG
AATGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)

AAA domain (AAA_19)

(217-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.191

100

0.982