Detailed information NTG0350154

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: GKC13_RS02320
• Genome accession: NZ_CP046134
• Coordinates: 432547..434787 (+)
• Length: 746 a.a.
• NCBI ID: WP_180479499.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain MAG_rmk202_sterm
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 427547..439787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC13_RS02295 (GKC13_02305)	-	428921..429190 (-)	270	WP_011226404.1	GIY-YIG nuclease family protein	-
GKC13_RS02300 (GKC13_02310)	-	429194..429958 (-)	765	WP_014608617.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
GKC13_RS09870	-	430008..430157 (-)	150	WP_014608616.1	polysaccharide deacetylase	-
GKC13_RS09875 (GKC13_02320)	-	430266..430799 (-)	534	WP_014608615.1	polysaccharide deacetylase family protein	-
GKC13_RS02310 (GKC13_02325)	-	430999..431754 (+)	756	WP_179970604.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
GKC13_RS02315 (GKC13_02330)	comEA	431862..432557 (+)	696	WP_014608614.1	helix-hairpin-helix domain-containing protein	Machinery gene
GKC13_RS02320 (GKC13_02335)	comEC/celB	432547..434787 (+)	2241	WP_180479499.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GKC13_RS02325 (GKC13_02340)	-	434873..436135 (+)	1263	WP_014608612.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
GKC13_RS02330 (GKC13_02345)	-	436201..436746 (+)	546	WP_014608611.1	GNAT family N-acetyltransferase	-
GKC13_RS02335 (GKC13_02350)	spxR	436739..438019 (+)	1281	WP_014608610.1	CBS-HotDog domain-containing transcription factor SpxR	-
GKC13_RS02340 (GKC13_02355)	-	438063..438923 (+)	861	WP_011226395.1	methionyl aminopeptidase	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84851.80 Da
• Isoelectric Point: 9.9196

>NTDB_id=350154 GKC13_RS02320 WP_180479499.1 432547..434787(+) (comEC/celB) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MWLKKAPINLFSLALLIVALYFTIFVSNFYAIGTFVFLVICFLRHHWKNRAALKLVGLIGGFFLVYFLFLYHIAIMQDKQ
TPAEIHQVTLVADTLSVNGERLSAIGKSNGQTYQVFYRLKSDKEQHFFKTTSQTLVLKGKIKLSSATGQRNFQGFDYKSY
LASQGIYRIAQIERLEHAVTPKSISPIAFFHQLRRKALVHIQTHFPNPMRHYMTGLLFGYLDKEFDEQSQLYTSLGIIHL
FALSGMQVGFFLGWFRYGLLRLGLPKNYILAILLPFSIFYGLMTGWTASVLRSLIQSLLAECGIKKLNNMGITLLLFFLV
LPHFLLTVGGVLSCSYAFLLCLFNFEEMPSFKKSIYMSLVLSLGTLPFLTYYYGTFQPLSLILTAIFSLVFDSFLLPVLT
VLFTLSGMVIFSQFNTLFEWMEFFLTWVQSWGVQPLILGKPSLFQFVSMIFVLVLLFDFWKKPQFRISLLMIFSLLMVWV
KHPLINEVTVVDVGQGDSIFLRSMKGETVLIDVGGKVTFGSKEKWQEGSQTSNAEKTLIPYLQARGVSQIDYLVLTHTDT
DHIGDLEVVAKRFKIKKICVSEGALTKPSFAKRIRFLKCPVRTLKAGDKLPMMGSNLQVLYPNKIGDGGNNDSLVLYGKL
LGTSFLFTGDLEKEGEEELMTSYPNLKVRVLKAGHHGSKDSSSEAFLDQLKPSLALVSAGENNRYKHPNDETLERFKERH
IKVLRTDQDGAIRFKGWFQLSSETVR

Nucleotide

• Length: 2241 bp

>NTDB_id=350154 GKC13_RS02320 WP_180479499.1 432547..434787(+) (comEC/celB) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATGTGGCTTAAAAAAGCTCCAATCAATCTTTTTTCCCTAGCGCTTTTAATAGTTGCTCTTTATTTTACTATTTTTGTATC
TAATTTCTATGCTATTGGGACTTTTGTCTTTCTGGTGATATGTTTTTTGAGGCATCATTGGAAGAATAGAGCAGCTCTAA
AGTTGGTGGGACTTATAGGTGGTTTCTTTTTGGTCTACTTTTTATTTTTATACCATATAGCTATCATGCAAGATAAACAA
ACTCCTGCTGAAATTCATCAGGTGACACTGGTTGCAGATACGCTCTCAGTTAATGGTGAGCGATTATCAGCAATCGGGAA
GTCAAATGGACAAACCTATCAGGTCTTTTACCGGCTAAAATCTGATAAGGAGCAGCATTTTTTTAAGACTACTAGTCAAA
CGCTTGTTTTAAAAGGGAAAATAAAGTTGTCCTCGGCAACTGGTCAACGTAATTTTCAAGGCTTTGATTATAAGTCTTAT
CTAGCTAGTCAGGGCATTTATAGGATTGCTCAGATTGAGCGTCTGGAACATGCCGTAACCCCAAAATCGATATCTCCAAT
AGCTTTTTTTCATCAATTGAGGAGGAAGGCTCTAGTTCATATTCAGACGCATTTTCCTAATCCGATGAGACACTACATGA
CAGGACTGCTCTTTGGGTATTTGGACAAGGAGTTTGATGAGCAGAGTCAACTCTACACTAGCTTGGGGATTATTCATCTT
TTTGCGCTATCGGGTATGCAGGTTGGATTTTTTCTTGGCTGGTTTCGCTATGGACTTCTCCGTTTGGGGCTTCCCAAAAA
TTATATCCTTGCTATCTTATTACCTTTTTCGATTTTCTATGGCTTAATGACTGGTTGGACGGCTTCGGTCTTACGTTCTT
TGATTCAAAGCCTCTTGGCTGAGTGTGGTATTAAAAAACTGAACAATATGGGCATAACGCTACTTCTATTTTTTCTAGTC
TTGCCTCATTTTCTTTTAACGGTAGGAGGTGTTTTAAGTTGTTCTTATGCCTTCTTGTTGTGTTTATTTAATTTTGAGGA
GATGCCGTCCTTTAAAAAGTCAATTTATATGAGTTTAGTATTGAGTCTTGGGACTTTGCCTTTTTTGACCTACTATTATG
GAACTTTTCAACCATTGAGTTTGATTCTGACGGCAATCTTCTCTCTAGTTTTTGATAGCTTTCTCTTACCTGTCTTAACA
GTACTTTTTACACTTTCAGGAATGGTAATTTTTTCTCAATTTAACACACTTTTTGAATGGATGGAGTTCTTTTTGACTTG
GGTACAATCTTGGGGAGTCCAGCCATTGATTTTAGGAAAACCTAGCTTGTTTCAGTTTGTCTCAATGATATTTGTATTGG
TTTTGCTCTTTGATTTTTGGAAAAAGCCTCAGTTTAGAATATCCCTCTTGATGATTTTTAGTCTTTTGATGGTCTGGGTC
AAACATCCTTTAATAAATGAAGTAACGGTGGTCGACGTTGGTCAAGGAGATAGTATTTTTTTAAGGAGTATGAAAGGTGA
AACGGTCCTAATAGATGTAGGTGGCAAGGTGACTTTCGGCTCTAAGGAAAAATGGCAAGAGGGGAGCCAGACGAGTAATG
CGGAGAAAACCTTAATTCCCTATCTACAGGCAAGGGGAGTGTCTCAAATTGACTATCTGGTTCTGACTCATACGGATACA
GACCATATTGGTGATTTGGAGGTAGTGGCTAAACGGTTTAAGATTAAGAAAATTTGTGTCAGTGAGGGGGCTTTGACTAA
GCCTAGTTTTGCCAAACGTATTCGATTTCTTAAATGTCCCGTTCGCACTTTAAAGGCTGGTGATAAGCTGCCTATGATGG
GAAGTAACCTACAGGTTCTTTATCCCAATAAAATTGGTGATGGTGGTAACAATGATTCACTTGTTCTCTATGGAAAGTTA
TTGGGAACCAGTTTTCTATTTACTGGTGATTTGGAAAAAGAAGGAGAAGAGGAATTGATGACTAGCTATCCAAATTTAAA
AGTAAGGGTCCTCAAAGCAGGACATCACGGTTCTAAAGACTCTTCATCAGAAGCTTTTTTGGACCAGCTAAAGCCATCAC
TTGCTCTTGTCTCAGCAGGTGAAAATAATCGTTATAAGCATCCAAATGATGAAACATTAGAGCGTTTCAAAGAACGTCAC
ATTAAGGTTTTACGCACAGACCAGGATGGTGCCATTCGGTTTAAGGGATGGTTCCAACTATCAAGTGAAACTGTCCGATA
A

Domains

Predicted by InterProScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-699)

• Domain of unknown function (DUF4131) (DUF4131) (29-170)

• Competence protein (Competence) (216-462)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	47.587	100	0.476
comEC/celB	Streptococcus mitis SK321	46.72	100	0.468
comEC/celB	Streptococcus pneumoniae TIGR4	45.783	100	0.458
comEC/celB	Streptococcus pneumoniae Rx1	45.515	100	0.456
comEC/celB	Streptococcus pneumoniae D39	45.515	100	0.456
comEC/celB	Streptococcus pneumoniae R6	45.515	100	0.456
comEC	Lactococcus lactis subsp. cremoris KW2	44.966	97.185	0.437