Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EYA82_RS18725 Genome accession   NZ_CP037758
Coordinates   530141..531460 (-) Length   439 a.a.
NCBI ID   WP_028358576.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain BPs115     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525141..536460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYA82_RS18705 (EYA82_18705) - 526040..526435 (-) 396 WP_004530943.1 nitrite reductase (NAD(P)H) small subunit -
  EYA82_RS18710 (EYA82_18710) nirB 526511..528955 (-) 2445 WP_004195264.1 nitrite reductase large subunit NirB -
  EYA82_RS18715 (EYA82_18715) - 529002..529343 (+) 342 WP_076829468.1 hypothetical protein -
  EYA82_RS18720 (EYA82_18720) maiA 529340..529984 (+) 645 WP_004548087.1 maleylacetoacetate isomerase -
  EYA82_RS18725 (EYA82_18725) pilA 530141..531460 (-) 1320 WP_028358576.1 signal recognition particle-docking protein FtsY Machinery gene
  EYA82_RS18730 (EYA82_18730) rsmD 531712..532338 (+) 627 WP_004522861.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EYA82_RS18740 (EYA82_18740) coaD 532449..532949 (+) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  EYA82_RS18745 (EYA82_18745) - 533012..533278 (+) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  EYA82_RS18750 (EYA82_18750) hisC 533448..534518 (-) 1071 WP_004553797.1 histidinol-phosphate transaminase -
  EYA82_RS18755 (EYA82_18755) pth 534607..535212 (-) 606 WP_004202941.1 aminoacyl-tRNA hydrolase -
  EYA82_RS18760 (EYA82_18760) - 535326..535940 (-) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46384.69 Da        Isoelectric Point: 6.0733

>NTDB_id=349926 EYA82_RS18725 WP_028358576.1 530141..531460(-) (pilA) [Burkholderia pseudomallei strain BPs115]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLGALREKVRAGRLTDPQQVKDALRELLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=349926 EYA82_RS18725 WP_028358576.1 530141..531460(-) (pilA) [Burkholderia pseudomallei strain BPs115]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAGCCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCATCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGATGCGCTGCGCGAGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCCGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-219)

SRP54-type protein, GTPase domain (SRP54)

(240-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.339

100

0.508

Multiple sequence alignment