Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E0Z08_RS03700 Genome accession   NZ_CP037424
Coordinates   788491..789612 (-) Length   373 a.a.
NCBI ID   WP_010326019.1    Uniprot ID   A0A1T1JGA2
Organism   Acinetobacter johnsonii strain M19     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783491..794612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0Z08_RS03670 (E0Z08_03670) - 784107..785075 (-) 969 WP_014538385.1 IS30 family transposase -
  E0Z08_RS03675 (E0Z08_03675) - 785432..785887 (+) 456 WP_005400160.1 bacteriohemerythrin -
  E0Z08_RS03680 (E0Z08_03680) - 786030..787112 (+) 1083 WP_029574954.1 membrane protein -
  E0Z08_RS03685 (E0Z08_03685) - 787112..787435 (+) 324 WP_010326018.1 RnfH family protein -
  E0Z08_RS03690 (E0Z08_03690) - 787482..787880 (-) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  E0Z08_RS03695 (E0Z08_03695) fur 787993..788430 (+) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  E0Z08_RS03700 (E0Z08_03700) pilU 788491..789612 (-) 1122 WP_010326019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E0Z08_RS03705 (E0Z08_03705) pilT 789639..790676 (-) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  E0Z08_RS03710 (E0Z08_03710) - 790799..791488 (+) 690 WP_058952300.1 YggS family pyridoxal phosphate-dependent enzyme -
  E0Z08_RS03715 (E0Z08_03715) - 791673..792407 (+) 735 WP_058952299.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41863.14 Da        Isoelectric Point: 6.6664

>NTDB_id=349685 E0Z08_RS03700 WP_010326019.1 788491..789612(-) (pilU) [Acinetobacter johnsonii strain M19]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=349685 E0Z08_RS03700 WP_010326019.1 788491..789612(-) (pilU) [Acinetobacter johnsonii strain M19]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCAGATTTATTTATCACCGCTGATGTTGAGCCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGACAACTGCTCAACTCAA
TTATGAGTGAAAAACAGCGTAAAGAATTTGCAGACACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCATTTCAACAGCGTGATATGCCAGGTATGGTTTTACGTCGGATTGAGACGCAAATTCCAACAAT
GGATGAGTTAAAACTTCCGCCTGTACTCAAAGAGTTAGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACGGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCAAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACGGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAAGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTAATTCCAACACCTGACGGTAATTCACGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGATCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
TTTACAAATCAAGTTGTCCGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T1JGA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

55.135

99.196

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362

Multiple sequence alignment