Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EYF70_RS23555 Genome accession   NZ_CP036401
Coordinates   5689010..5690695 (+) Length   561 a.a.
NCBI ID   WP_174800425.1    Uniprot ID   A0AA88C3Q1
Organism   Pseudoduganella albidiflava strain DSM 17472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5684010..5695695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYF70_RS23540 (EYF70_23540) xdhA 5684075..5685553 (-) 1479 WP_131147561.1 xanthine dehydrogenase small subunit -
  EYF70_RS23545 (EYF70_23545) - 5685679..5687220 (-) 1542 WP_131147562.1 carboxylesterase/lipase family protein -
  EYF70_RS23550 (EYF70_23550) - 5687562..5688833 (+) 1272 WP_131147563.1 HlyC/CorC family transporter -
  EYF70_RS23555 (EYF70_23555) pilF 5689010..5690695 (+) 1686 WP_174800425.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EYF70_RS23560 (EYF70_23560) pilC 5690718..5691929 (+) 1212 WP_131147565.1 type II secretion system F family protein Machinery gene
  EYF70_RS23565 (EYF70_23565) - 5691965..5693659 (-) 1695 WP_131147566.1 SEC-C metal-binding domain-containing protein -
  EYF70_RS23570 (EYF70_23570) - 5693757..5695106 (-) 1350 WP_218943718.1 MFS transporter -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 60598.16 Da        Isoelectric Point: 6.0814

>NTDB_id=348782 EYF70_RS23555 WP_174800425.1 5689010..5690695(+) (pilF) [Pseudoduganella albidiflava strain DSM 17472]
MPGLARVLVQAGRLTTAQADALQKRAGTDKQPFIDVLLGSGIIGARDLAAFCSETFAYPMLDLHAISVAALPHKIIDEKL
MQSGRVVALAKRGNKMSVAISDPTNTQALDQIKFQTESAVEPVIVPHDALLRLLHELGKSSAQLMDELAGAESEIQFAED
QEAAATEAPATDIEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLVEHAQPPIAIKDKLVSRIKVLARL
DISEKRVPQDGRMRLIVSPTKTLDLRISTLPTLFGEKTVMRILDATQAQMGIDALGYEPEQRALLLDAIQRPYGMVLVTG
PTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIMVGEIRDLETADIA
IKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCGCKQPLDIAEDKLISAGFRAEEVDGSW
KPYGPVGCERCNGSGYKGRVGIYQIMPISPAIEALILAHGNAMQIAAQAQAEGVKSLRQSGLVKVKAGLTSLEEVLGCTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=348782 EYF70_RS23555 WP_174800425.1 5689010..5690695(+) (pilF) [Pseudoduganella albidiflava strain DSM 17472]
ATGCCGGGACTGGCACGGGTCCTGGTACAGGCCGGCCGCCTGACGACCGCGCAGGCGGATGCGCTGCAAAAGCGCGCGGG
CACGGACAAGCAGCCCTTCATCGACGTGCTGCTCGGCAGCGGCATCATCGGCGCGCGCGACCTGGCGGCCTTCTGCTCCG
AAACGTTCGCCTACCCGATGCTGGATCTGCACGCGATCAGCGTGGCCGCGCTGCCCCACAAGATCATCGACGAAAAACTG
ATGCAGAGCGGCCGCGTGGTGGCGCTGGCCAAGCGGGGCAACAAGATGTCGGTGGCGATTTCCGATCCCACCAACACGCA
GGCGCTGGACCAGATCAAGTTCCAGACCGAATCGGCGGTGGAACCGGTGATCGTGCCGCACGATGCGCTGTTGCGGCTGC
TGCACGAGCTCGGCAAGAGCAGCGCCCAGCTGATGGACGAACTGGCCGGCGCGGAAAGCGAGATCCAGTTCGCCGAAGAC
CAGGAAGCGGCGGCAACGGAGGCGCCGGCCACCGATATCGAGGATGCGCCGATCGTGCGCTTCCTGAACAAGATGCTGAT
GGATGCCGTGAACATGGGCGCCTCCGACCTGCACTTCGAGCCGTTCGAGAAGTTCTACCGGATCCGCTTCCGCGTCGACG
GCGTGCTGGTCGAACACGCGCAGCCGCCGATCGCCATCAAGGACAAGCTGGTATCGCGCATCAAGGTGCTGGCCCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGATGGCCGCATGCGGCTGATCGTGTCGCCCACCAAGACGCTCGACCTGCGTAT
CTCCACGCTGCCAACGCTGTTCGGCGAGAAGACCGTGATGCGTATCCTCGACGCCACGCAGGCGCAGATGGGCATCGATG
CGCTGGGCTACGAACCGGAACAGCGCGCGCTGCTGCTCGATGCGATCCAGCGTCCGTACGGCATGGTGCTGGTCACGGGC
CCCACCGGTTCGGGCAAGACGGTGTCGCTGTACACCTGCCTGAACATCCTGAACAAGCCGGGCATCAATATCTCGACGGC
GGAAGATCCGGCCGAGATCAACCTGCCCGGCGTGAACCAGGTGAACGTGAACGACAAGGCCGGGCTGACCTTCCCGGTGG
CGCTGAAATCGTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGATATCGCG
ATCAAGGCGGCGCAGACGGGCCACATGGTGTTCTCCACGCTGCACACCAACGATGCGCCCTCCACGCTGACGCGCCTGAT
GAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGATCCTGATCACGGCGCAGCGGCTGGCGCGCCGGCTGTGCG
GCTGCAAGCAGCCGCTGGACATCGCCGAGGACAAGCTCATCAGCGCCGGTTTCCGCGCCGAGGAAGTCGACGGCAGCTGG
AAGCCGTACGGCCCGGTGGGCTGCGAACGGTGCAACGGCTCCGGCTACAAGGGGCGCGTGGGCATCTACCAGATCATGCC
CATCAGCCCGGCCATCGAGGCGCTGATCCTGGCGCACGGCAACGCGATGCAGATCGCCGCCCAGGCCCAGGCCGAAGGCG
TGAAATCGCTGCGCCAGTCCGGCCTGGTGAAAGTGAAGGCGGGATTGACCAGCCTGGAAGAAGTGCTGGGCTGCACCAAC
GAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.064

100

0.545
  pilB Acinetobacter baumannii D1279779

53.108

100

0.533
  pilB Acinetobacter baylyi ADP1

52.941

100

0.529
  pilB Legionella pneumophila strain ERS1305867

48.841

100

0.488
  pilB Vibrio cholerae strain A1552

45.534

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.815

100

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

44.405

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.83

100

0.39
  pilF Thermus thermophilus HB27

38.475

98.217

0.378

Multiple sequence alignment