Detailed information NTG0348053

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C3E96_RS25155
• Genome accession: NZ_CP036327
• Coordinates: 5056488..5057288 (-)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain BA28434
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5051488..5062288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3E96_RS25135 (C3E96_025135)	dksA	5052068..5052523 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
C3E96_RS25140 (C3E96_025140)	sfsA	5052686..5053402 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
C3E96_RS25145 (C3E96_025145)	thpR	5053402..5053938 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
C3E96_RS25150 (C3E96_025150)	hrpB	5054011..5056440 (+)	2430	WP_131086829.1	ATP-dependent helicase HrpB	-
C3E96_RS25155 (C3E96_025155)	pilD	5056488..5057288 (-)	801	WP_004191960.1	prepilin peptidase	Machinery gene
C3E96_RS25160 (C3E96_025160)	-	5057285..5058031 (-)	747	WP_023282189.1	type II secretion system protein N	-
C3E96_RS25165 (C3E96_025165)	-	5058021..5058506 (-)	486	WP_004145896.1	type II secretion system protein M	-
C3E96_RS25170 (C3E96_025170)	gspL	5058499..5059692 (-)	1194	WP_004191957.1	type II secretion system protein GspL	-
C3E96_RS25175 (C3E96_025175)	gspK	5059679..5060665 (-)	987	WP_077265688.1	type II secretion system minor pseudopilin GspK	-
C3E96_RS25180 (C3E96_025180)	gspJ	5060662..5061258 (-)	597	WP_064162459.1	type II secretion system minor pseudopilin GspJ	-
C3E96_RS25185 (C3E96_025185)	gspI	5061255..5061620 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
C3E96_RS25190 (C3E96_025190)	gspH	5061617..5062132 (-)	516	WP_062920974.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348053 C3E96_RS25155 WP_004191960.1 5056488..5057288(-) (pilD) [Klebsiella pneumoniae strain BA28434]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=348053 C3E96_RS25155 WP_004191960.1 5056488..5057288(-) (pilD) [Klebsiella pneumoniae strain BA28434]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421