Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW56_RS20300 Genome accession   NZ_CP036132
Coordinates   3961202..3962245 (-) Length   347 a.a.
NCBI ID   WP_215596894.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS823     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3956202..3967245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW56_RS20255 (EXW56_20665) - 3956602..3956802 (-) 201 WP_002015009.1 YqzE family protein -
  EXW56_RS20260 (EXW56_20670) - 3956841..3957338 (-) 498 WP_002111816.1 shikimate kinase -
  EXW56_RS20265 (EXW56_20675) - 3957490..3958140 (-) 651 WP_002199367.1 2OG-Fe(II) oxygenase -
  EXW56_RS20270 (EXW56_20680) comGG 3958313..3958684 (-) 372 WP_002199366.1 competence type IV pilus minor pilin ComGG -
  EXW56_RS20275 (EXW56_20685) comGF 3958681..3959142 (-) 462 WP_215557464.1 competence type IV pilus minor pilin ComGF -
  EXW56_RS20280 (EXW56_20690) - 3959126..3959422 (-) 297 WP_002111824.1 hypothetical protein -
  EXW56_RS20285 (EXW56_20695) comGD 3959415..3959870 (-) 456 WP_002199363.1 competence type IV pilus minor pilin ComGD -
  EXW56_RS20290 (EXW56_20700) comGC 3959867..3960166 (-) 300 WP_002111826.1 competence type IV pilus major pilin ComGC -
  EXW56_RS20295 (EXW56_20705) comGB 3960178..3961215 (-) 1038 WP_002199362.1 competence type IV pilus assembly protein ComGB -
  EXW56_RS20300 (EXW56_20710) comGA 3961202..3962245 (-) 1044 WP_215596894.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW56_RS20305 (EXW56_20715) - 3962451..3963146 (+) 696 WP_002199360.1 helix-turn-helix domain-containing protein -
  EXW56_RS20310 (EXW56_20720) - 3963278..3963520 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW56_RS20315 (EXW56_20725) - 3963627..3965021 (+) 1395 WP_002199359.1 L-cystine transporter -
  EXW56_RS20320 (EXW56_20730) - 3965102..3965512 (-) 411 WP_002199358.1 hypothetical protein -
  EXW56_RS20325 (EXW56_20735) - 3965582..3965797 (-) 216 WP_002199357.1 DUF3912 family protein -
  EXW56_RS20330 (EXW56_20740) - 3966050..3966361 (+) 312 WP_002199356.1 hypothetical protein -
  EXW56_RS20335 (EXW56_20745) - 3966395..3966883 (-) 489 WP_002111841.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39130.33 Da        Isoelectric Point: 8.2369

>NTDB_id=347037 EXW56_RS20300 WP_215596894.1 3961202..3962245(-) (comGA) [Bacillus mycoides strain JAS823]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQSSVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDATGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=347037 EXW56_RS20300 WP_215596894.1 3961202..3962245(-) (comGA) [Bacillus mycoides strain JAS823]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAATCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCTAGAAAAGGAGAAACACGTCGTATTGTTACTCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGACAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment