Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW54_RS20940 Genome accession   NZ_CP036094
Coordinates   4158837..4159880 (-) Length   347 a.a.
NCBI ID   WP_001013172.1    Uniprot ID   -
Organism   Bacillus toyonensis strain JAS22/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4153837..4164880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW54_RS20895 (EXW54_21400) - 4154263..4154463 (-) 201 WP_000106086.1 YqzE family protein -
  EXW54_RS20900 (EXW54_21405) - 4154502..4154999 (-) 498 WP_000836652.1 shikimate kinase -
  EXW54_RS20905 (EXW54_21410) - 4155119..4155769 (-) 651 WP_000183875.1 2OG-Fe(II) oxygenase -
  EXW54_RS20910 (EXW54_21415) comGG 4155944..4156315 (-) 372 WP_000743702.1 competence type IV pilus minor pilin ComGG -
  EXW54_RS20915 (EXW54_21420) comGF 4156312..4156785 (-) 474 WP_000477944.1 competence type IV pilus minor pilin ComGF -
  EXW54_RS20920 (EXW54_21425) - 4156755..4157057 (-) 303 WP_000829525.1 hypothetical protein -
  EXW54_RS20925 (EXW54_21430) comGD 4157050..4157505 (-) 456 WP_000810404.1 competence type IV pilus minor pilin ComGD -
  EXW54_RS20930 (EXW54_21435) comGC 4157502..4157801 (-) 300 WP_098812018.1 competence type IV pilus major pilin ComGC -
  EXW54_RS20935 (EXW54_21440) comGB 4157813..4158850 (-) 1038 WP_002093281.1 competence type IV pilus assembly protein ComGB -
  EXW54_RS20940 (EXW54_21445) comGA 4158837..4159880 (-) 1044 WP_001013172.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW54_RS20945 (EXW54_21450) - 4160085..4160780 (+) 696 WP_000434112.1 helix-turn-helix domain-containing protein -
  EXW54_RS20950 (EXW54_21455) - 4160906..4161148 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  EXW54_RS20955 (EXW54_21460) - 4161256..4162650 (+) 1395 WP_001094349.1 L-cystine transporter -
  EXW54_RS20960 (EXW54_21465) - 4162713..4163105 (-) 393 WP_000875408.1 hypothetical protein -
  EXW54_RS20965 (EXW54_21470) - 4163193..4163408 (-) 216 WP_001008324.1 DUF3912 family protein -
  EXW54_RS20970 (EXW54_21475) - 4163661..4163972 (+) 312 WP_001092833.1 hypothetical protein -
  EXW54_RS20975 (EXW54_21480) - 4164007..4164495 (-) 489 WP_098058926.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39204.57 Da        Isoelectric Point: 8.5491

>NTDB_id=346690 EXW54_RS20940 WP_001013172.1 4158837..4159880(-) (comGA) [Bacillus toyonensis strain JAS22/1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLITKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYRESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKRKTRRIVTLEDPVEKRKDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQALLAVAAQRLVELKCPFCRGKCSILCKSMRQVRQASVYELLDGYDLQQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346690 EXW54_RS20940 WP_001013172.1 4158837..4159880(-) (comGA) [Bacillus toyonensis strain JAS22/1]
ATGAATGGAATTGAAATTTTTGCGAATACAATTTTGAAAGAAGCGTGTAGGGTACAAGCATCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAGCTACGTATAGGAAAAGATTTAATTACGAAACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCGCACTTTAAATTTTTGGCATCCATGGATATAGGAGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATTGATGGACAAGAAGTGTATTTACGTCTTTCAACACTTCCGACTGTATATCGAGAAAGTCTCGTGATTCGCCT
TCATTTACAAGCATCTGTGCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACGGCGGAAAAACTCCTCTCTTTTTTAC
GTTATTCACATGGTCTACTCGTATTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAGGAAAACACGTCGAATTGTAACACTTGAAGACCCAGTTGAAAAAAGAAAAGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTACGCCATGATCCAGATATTATATTAGTTG
GTGAAATCCGGGATGAAGAAACAGCAAAAATAGCTGTAAGAGCAAGTTTAACTGGACATTTAGTAATGACGACCCTGCAT
ACGAATGATGCGAAAGGAGCGATACTCAGATTTATGGATTATGGCATAACGAGGCAAGAAATTGAACAAGCTTTATTGGC
TGTAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCATTTTGCAGAGGAAAGTGCTCAATTTTATGTAAATCAATGCGGC
AAGTAAGACAAGCAAGCGTTTACGAGTTGTTAGATGGATATGATTTGCAACAAGCAATTAAAGAAGCGAACGGAGAATGT
GTTACGTACAAACATGAAACATTAGAATCTTCGATACGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576

Multiple sequence alignment