Detailed information NTG0346470

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EXW45_RS05965
• Genome accession: NZ_CP036073
• Coordinates: 1164997..1168722 (+)
• Length: 1241 a.a.
• NCBI ID: WP_149294166.1
• Uniprot ID: -
• Organism: Bacillus wiedmannii strain JAS08/1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1159997..1173722

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW45_RS05950 (EXW45_06020)	-	1160015..1160608 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
EXW45_RS05955 (EXW45_06025)	lepB	1160805..1161368 (+)	564	WP_048567025.1	signal peptidase I	-
EXW45_RS05960 (EXW45_06030)	addB	1161485..1165000 (+)	3516	WP_097994408.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EXW45_RS05965 (EXW45_06035)	addA	1164997..1168722 (+)	3726	WP_149294166.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EXW45_RS05970 (EXW45_06040)	-	1168735..1169022 (+)	288	WP_001240558.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
EXW45_RS05975 (EXW45_06045)	gerPF	1169104..1169319 (-)	216	WP_001141570.1	spore germination protein GerPF	-
EXW45_RS05980 (EXW45_06050)	-	1169362..1169748 (-)	387	WP_046958325.1	spore germination protein GerPE	-
EXW45_RS05985 (EXW45_06055)	gerPD	1169764..1169958 (-)	195	WP_001052804.1	spore germination protein GerPD	-
EXW45_RS05990 (EXW45_06060)	gerPC	1169965..1170579 (-)	615	WP_001070746.1	spore germination protein GerPC	-
EXW45_RS05995 (EXW45_06065)	gerPB	1170648..1170854 (-)	207	WP_001012513.1	spore germination protein GerPB	-
EXW45_RS06000 (EXW45_06070)	gerPA	1170869..1171090 (-)	222	WP_001111187.1	spore germination protein GerPA	-
EXW45_RS06005 (EXW45_06075)	-	1171184..1171363 (-)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
EXW45_RS06010 (EXW45_06080)	-	1171610..1172509 (+)	900	WP_179953655.1	fumarylacetoacetate hydrolase family protein	-
EXW45_RS06015 (EXW45_06085)	-	1172545..1172829 (-)	285	WP_046958323.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142791.02 Da
• Isoelectric Point: 4.8348

>NTDB_id=346470 EXW45_RS05965 WP_149294166.1 1164997..1168722(+) (addA) [Bacillus wiedmannii strain JAS08/1]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAARESWTSVYEAMQNISWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYEYDTNWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=346470 EXW45_RS05965 WP_149294166.1 1164997..1168722(+) (addA) [Bacillus wiedmannii strain JAS08/1]
ATGATTGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATATTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCGGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTCGTGAGTCATGGACAAGCGTTTATGAAGCGATGCAAAACATATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGACATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGTGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGCTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTTATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTAGGTGGCGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTGAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCGGAAGTAGAAAAAGCACAACTTGAAGCTCGCCTTATG
GCGCAGCGAATAAAGGCTATGGTTGATTCAGGATATACCGTCTATGATCGCAAAACGAATGAAATGCGACAAGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGGTTAAATGATGAGGAACTTGCGACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACTGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATGAATATGACACGAACTGGAAA
GTGGAAGTTGTTGACGGCAAAACGTTACTTGCACCGGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGATTGACGTACGCAGAGCGTGG
GACAGCAGTCCATGCTGTTATGCAACATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAGCAAATTGCCG
GAATGGTAAATAAGGAACTATTAACATTCGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGATGAAACAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTCAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAGGCGCTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTCAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534