Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW45_RS05960 Genome accession   NZ_CP036073
Coordinates   1161485..1165000 (+) Length   1171 a.a.
NCBI ID   WP_097994408.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain JAS08/1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1156485..1170000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW45_RS05930 (EXW45_05995) - 1157036..1157827 (-) 792 WP_048567023.1 phosphotransferase -
  EXW45_RS05935 (EXW45_06000) cspA 1158201..1158404 (+) 204 WP_000301521.1 RNA chaperone/antiterminator CspA -
  EXW45_RS05940 (EXW45_06005) - 1158656..1158841 (-) 186 WP_000391437.1 hypothetical protein -
  EXW45_RS05945 (EXW45_06010) - 1159125..1159706 (+) 582 WP_048567024.1 competence protein ComK -
  EXW45_RS05950 (EXW45_06020) - 1160015..1160608 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  EXW45_RS05955 (EXW45_06025) lepB 1160805..1161368 (+) 564 WP_048567025.1 signal peptidase I -
  EXW45_RS05960 (EXW45_06030) addB 1161485..1165000 (+) 3516 WP_097994408.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW45_RS05965 (EXW45_06035) addA 1164997..1168722 (+) 3726 WP_149294166.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW45_RS05970 (EXW45_06040) - 1168735..1169022 (+) 288 WP_001240558.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW45_RS05975 (EXW45_06045) gerPF 1169104..1169319 (-) 216 WP_001141570.1 spore germination protein GerPF -
  EXW45_RS05980 (EXW45_06050) - 1169362..1169748 (-) 387 WP_046958325.1 spore germination protein GerPE -
  EXW45_RS05985 (EXW45_06055) gerPD 1169764..1169958 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134300.49 Da        Isoelectric Point: 5.2494

>NTDB_id=346469 EXW45_RS05960 WP_097994408.1 1161485..1165000(+) (addB) [Bacillus wiedmannii strain JAS08/1]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSAYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWISEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDKFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSAKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346469 EXW45_RS05960 WP_097994408.1 1161485..1165000(+) (addB) [Bacillus wiedmannii strain JAS08/1]
ATGTCACTTCGATTTGTAATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
GCAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATCGGGA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGCTAGTCGAAAAGCTTCCACAGTCGGCATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGCGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCCCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGATTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACAGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGATGTTCTTAGTGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATAAATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATATGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGACATTTTCAAATTGGATGC
GCCAGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGGTCGCTTCCGCCGATGG
AGTTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCTTACGTATTATTGACTATAAATCCAGTGCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGGACAGCATCACCAGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGGTGAAAGGTGACGCATCGGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGACTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCGAGTATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492

Multiple sequence alignment