Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   EWI30_RS00745 Genome accession   NZ_CP035738
Coordinates   159192..160712 (-) Length   506 a.a.
NCBI ID   WP_129994743.1    Uniprot ID   -
Organism   Enterobacter cloacae strain CZ-1     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 154192..165712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWI30_RS00735 (EWI30_00735) hdfR 157888..158709 (-) 822 WP_129994742.1 HTH-type transcriptional regulator HdfR -
  EWI30_RS00740 (EWI30_00740) - 158828..159166 (+) 339 WP_008501577.1 DUF413 domain-containing protein -
  EWI30_RS00745 (EWI30_00745) comM 159192..160712 (-) 1521 WP_129994743.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  EWI30_RS00750 (EWI30_00750) ilvL 161053..161151 (+) 99 WP_001311244.1 ilv operon leader peptide -
  EWI30_RS00755 (EWI30_00755) ilvX 161238..161288 (+) 51 WP_108091188.1 peptide IlvX -
  EWI30_RS00760 (EWI30_00760) ilvG 161291..162937 (+) 1647 WP_114979850.1 acetolactate synthase 2 catalytic subunit -
  EWI30_RS00765 (EWI30_00765) ilvM 162934..163197 (+) 264 WP_129994744.1 acetolactate synthase 2 small subunit -
  EWI30_RS00770 (EWI30_00770) ilvE 163216..164145 (+) 930 WP_021242827.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55195.22 Da        Isoelectric Point: 7.0037

>NTDB_id=344147 EWI30_RS00745 WP_129994743.1 159192..160712(-) (comM) [Enterobacter cloacae strain CZ-1]
MSLSVVYTRAAIGVKAPLISVEVHLSNGLPGLTLVGLPETTVKEARDRVRSAIINSGYTFPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLNASRLGSYEFIGELALTGALRGVPGAISGALEAIRAGRQIIVANENGSEVSLIAEKGCLIAGHL
QEVCAYLEGRHELSEPEETGELLPESLDDLSDIIGQEQGKRALEITAAGGHNLLLIGPPGTGKTMLASRLNGLLPPLNNH
EALESAAIFSLVSSVSLQKQWRRRPFRSPHHSASLTAMVGGGSIPGPGEISLAHNGILFLDELPEFERRVLDALREPIES
GEIHISRTRAKISYPAQFQLVAAMNPSPSGHYQGNHNRCTPEQTLRYLGKLSGPFLDRFDLSLEIPLPPPGLLSQSSVRG
ESTVEVRERVIAAQARQYIRQNKLNARLDNAEIRQFCQLKAEDANWLEETLTRFGLSIRAWQRLLKVARTIADVERCHEI
ERQHLQEALSYRAIDRLLLHLQKLLA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=344147 EWI30_RS00745 WP_129994743.1 159192..160712(-) (comM) [Enterobacter cloacae strain CZ-1]
ATGTCACTGTCAGTTGTTTATACGCGTGCGGCTATTGGAGTCAAAGCACCGCTGATTTCCGTAGAGGTCCATCTGAGCAA
TGGGCTTCCCGGATTAACACTCGTCGGTTTGCCGGAAACAACGGTGAAAGAAGCCAGAGATCGGGTACGCAGCGCAATCA
TCAATAGCGGTTATACCTTCCCCGCAAAGAAGATCACTATCAACCTGGCGCCTGCGGACCTGCCCAAAGAGGGGGGACGA
TATGATTTACCTATCGCTATAGCGCTTCTCGCTGCCTCTGAACAGCTTAATGCATCCAGGCTAGGCTCGTATGAGTTCAT
CGGTGAACTGGCGCTTACAGGGGCATTGAGAGGCGTTCCTGGCGCAATCTCAGGTGCGCTGGAAGCCATTCGTGCTGGTC
GACAAATCATTGTTGCCAATGAAAATGGTTCCGAGGTGAGCCTGATAGCCGAAAAAGGATGCCTGATTGCAGGGCACTTG
CAGGAGGTTTGCGCATATCTTGAGGGGCGACATGAGTTGTCAGAGCCTGAAGAAACAGGGGAGCTCCTACCTGAAAGCCT
GGACGATCTCAGCGATATCATCGGTCAGGAGCAGGGGAAACGGGCACTGGAGATTACCGCTGCCGGAGGGCATAACCTGC
TGTTGATTGGTCCGCCAGGTACTGGGAAAACGATGCTGGCGAGCAGATTGAATGGTCTGCTGCCCCCACTCAATAACCAT
GAAGCACTTGAAAGCGCTGCAATATTTAGCCTGGTGAGTTCTGTGTCGTTACAAAAACAGTGGCGACGTCGCCCTTTCCG
CTCTCCGCACCACAGTGCCTCACTAACAGCCATGGTCGGCGGAGGCTCTATACCGGGGCCGGGAGAGATCTCACTGGCCC
ACAATGGCATTCTGTTTCTTGATGAGCTGCCCGAGTTTGAAAGGCGCGTGCTGGATGCTCTGAGGGAGCCTATCGAATCG
GGTGAAATTCATATCTCCCGTACACGTGCCAAAATAAGCTATCCCGCGCAGTTCCAGCTGGTTGCAGCAATGAACCCGAG
CCCGTCAGGACATTATCAGGGTAATCATAATCGCTGTACGCCTGAGCAGACGCTGCGATACCTGGGGAAACTCTCTGGCC
CTTTTCTGGATCGTTTCGATTTATCCCTGGAGATCCCTTTGCCTCCCCCTGGCCTGCTCAGCCAGAGTAGCGTCAGAGGG
GAGAGCACGGTAGAGGTTCGCGAGCGGGTTATCGCCGCTCAAGCCCGGCAGTATATACGTCAAAATAAACTGAATGCCCG
TCTGGATAATGCTGAGATTCGGCAGTTTTGCCAGCTAAAGGCTGAGGACGCAAACTGGCTGGAAGAAACATTGACGCGAT
TTGGGCTCTCAATTCGTGCGTGGCAGCGTCTCTTAAAAGTCGCCCGAACTATCGCCGATGTAGAAAGGTGTCATGAAATT
GAGAGACAACATCTGCAGGAAGCGTTGAGCTATCGTGCAATCGACCGTCTGCTTTTGCACCTGCAAAAGTTACTGGCGTA
A

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.398

100

0.591
  comM Vibrio cholerae strain A1552

59.363

99.209

0.589
  comM Glaesserella parasuis strain SC1401

58.777

100

0.589
  comM Vibrio campbellii strain DS40M4

59.046

99.407

0.587
  comM Legionella pneumophila str. Paris

50.101

98.221

0.492
  comM Legionella pneumophila strain ERS1305867

50.101

98.221

0.492
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.181

100

0.443

Multiple sequence alignment