Detailed information NTG0332350

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: EKO22_RS06490
• Genome accession: NZ_CP034671
• Coordinates: 1325314..1326402 (-)
• Length: 362 a.a.
• NCBI ID: WP_208678881.1
• Uniprot ID: A0AAT9K0X3
• Organism: Synechococcus elongatus PCC 11802
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1320314..1331402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EKO22_RS06470 (EKO22_06470)	dusA	1321244..1322257 (-)	1014	WP_208679095.1	tRNA dihydrouridine(20/20a) synthase DusA	-
EKO22_RS06475 (EKO22_06475)	-	1322284..1323516 (+)	1233	WP_208678884.1	NAD(P)/FAD-dependent oxidoreductase	-
EKO22_RS06480 (EKO22_06480)	-	1323496..1324053 (-)	558	WP_208678883.1	hypothetical protein	-
EKO22_RS06485 (EKO22_06485)	-	1324058..1325290 (-)	1233	WP_208678882.1	type II secretion system F family protein	-
EKO22_RS06490 (EKO22_06490)	pilT	1325314..1326402 (-)	1089	WP_208678881.1	type IV pilus twitching motility protein PilT	Machinery gene
EKO22_RS06495 (EKO22_06495)	pilB/pilB1	1326474..1328474 (-)	2001	WP_208678880.1	GspE/PulE family protein	Machinery gene
EKO22_RS06500 (EKO22_06500)	grpE	1328751..1329374 (+)	624	WP_338438663.1	nucleotide exchange factor GrpE	-

Sequence

Protein

• Length: 362 a.a.
• Molecular weight: 39840.95 Da
• Isoelectric Point: 6.3643

>NTDB_id=332350 EKO22_RS06490 WP_208678881.1 1325314..1326402(-) (pilT) [Synechococcus elongatus PCC 11802]
MELMIEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLEQNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPEFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAIIELINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLVAVFSQTLVPKRSARPGSFGRVLAQEIMIVTPAVSNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAASKTSRPDEMQRLIGNVSIAA

Nucleotide

• Length: 1089 bp

>NTDB_id=332350 EKO22_RS06490 WP_208678881.1 1325314..1326402(-) (pilT) [Synechococcus elongatus PCC 11802]
ATGGAACTAATGATCGAAGACCTGATGGAAAAGCTCGTGGAGATGGGAGGATCTGACCTCCATCTTTCTGTTGGCTTACC
TCCTTATTTTCGAATCAGTGGAAAACTTACCCCCATCGGGGATGAACCAATGGGTGAGGAACAGTGTCAGCGCTTGATCT
TCAGTATGTTGAACAACACGCAACGCAAAATGCTGGAGCAAAATTGGGAGCTTGATTGTTCCTATGGGATTCGTGGACTC
GCTCGTTTCCGGGTCAATGTTTACAAAGATAAAGGAACCTATGCCGCTTGCTTACGGGCTTTGAGTGCCAAGGTGCCGGA
GTTTAACGACCTAGGGCTACCTCAAGTGGTGCGCGAGATGAGCGAAAAACCAAGGGGGCTCATTCTCGTCACTGGCCCTA
CAGGGTCTGGGAAAACAACAACGTTGGCAGCCATTATTGAGTTAATTAACCGCAGTCGAGCTGAACATATCATTACGGTT
GAAGATCCCATTGAGTTTGTCTATCAACCCGCAAAAAGCATCATTCACCAGCGACAGCTTGGAGAAGATACGAAGAGTTT
TGCTAATGCCCTGCGAGCAGCGCTTCGAGAAGATCCTGACATCATTCTTGTGGGTGAGATGCGGGACTTAGAAACGATTC
AGTTAGCTATTACTGCTGCAGAAACTGGTCACCTTGTTTTTGGTACTTTGCACACCAGCTCCGCTGCTCAAACCGTTGAC
CGGATTATCGATGTCTTTCCTGCTGAAAAACAGACGCAGGTAAGGGTTCAGTTGTCGAATTCACTAGTTGCGGTCTTCAG
TCAAACCCTTGTCCCCAAACGCAGCGCTCGACCTGGTAGCTTTGGTCGTGTCCTTGCCCAAGAGATCATGATTGTTACAC
CAGCAGTTTCCAACTTAATTCGCGAAGGAAAAACCGCACAAATTTACTCTCAAATTCAGACGGGCGGAAAACTAGGCATG
CAGACCCTAGAGAAAGCTTTGGCTGATCTTTATAAGGCAGGTGAAATCACTTTTGAAGCAGCTGCCTCTAAAACATCTAG
GCCCGATGAGATGCAACGGTTGATTGGCAATGTTTCAATTGCTGCCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (48-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas stutzeri DSM 10701	53.026	95.856	0.508
pilT	Pseudomonas aeruginosa PAK	52.738	95.856	0.506
pilT	Legionella pneumophila strain ERS1305867	53.043	95.304	0.506
pilT	Acinetobacter baylyi ADP1	53.043	95.304	0.506
pilT	Legionella pneumophila strain Lp02	53.043	95.304	0.506
pilT	Acinetobacter baumannii strain A118	52.174	95.304	0.497
pilT	Acinetobacter baumannii D1279779	52.174	95.304	0.497
pilT	Acinetobacter nosocomialis M2	52.174	95.304	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.296	98.066	0.483
pilT	Neisseria meningitidis 8013	50.143	96.409	0.483
pilT	Neisseria gonorrhoeae MS11	50.143	96.409	0.483
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.118	93.923	0.461
pilT	Vibrio cholerae strain A1552	49.118	93.923	0.461
pilU	Pseudomonas stutzeri DSM 10701	39.886	96.961	0.387
pilU	Acinetobacter baylyi ADP1	37.536	96.409	0.362