Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EJ998_RS16305 Genome accession   NZ_CP034553
Coordinates   3330377..3331882 (+) Length   501 a.a.
NCBI ID   WP_027789104.1    Uniprot ID   A0A104AWL9
Organism   Burkholderia cepacia ATCC 25416     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3325377..3336882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ998_RS16280 (EJ998_16280) kdsD 3326609..3327592 (+) 984 WP_027789106.1 arabinose 5-phosphate isomerase KdsD -
  EJ998_RS16285 (EJ998_16285) - 3327592..3328128 (+) 537 WP_021162596.1 HAD family hydrolase -
  EJ998_RS16290 (EJ998_16290) lptC 3328132..3328746 (+) 615 WP_027789105.1 LPS export ABC transporter periplasmic protein LptC -
  EJ998_RS16295 (EJ998_16295) lptA 3328767..3329432 (+) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  EJ998_RS16300 (EJ998_16300) lptB 3329429..3330205 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  EJ998_RS16305 (EJ998_16305) rpoN/rpoN1 3330377..3331882 (+) 1506 WP_027789104.1 RNA polymerase factor sigma-54 Machinery gene
  EJ998_RS16310 (EJ998_16310) raiA 3332041..3332400 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  EJ998_RS16315 (EJ998_16315) - 3332569..3333108 (+) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  EJ998_RS16320 (EJ998_16320) hprK 3333240..3334208 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  EJ998_RS16325 (EJ998_16325) rapZ 3334284..3335192 (+) 909 WP_021162602.1 RNase adapter RapZ -
  EJ998_RS16330 (EJ998_16330) - 3335220..3335855 (+) 636 WP_027789102.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54797.94 Da        Isoelectric Point: 4.8767

>NTDB_id=331901 EJ998_RS16305 WP_027789104.1 3330377..3331882(+) (rpoN/rpoN1) [Burkholderia cepacia ATCC 25416]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=331901 EJ998_RS16305 WP_027789104.1 3330377..3331882(+) (rpoN/rpoN1) [Burkholderia cepacia ATCC 25416]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TAGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGCAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCACTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTACGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.047

100

0.689

Multiple sequence alignment