Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EJN89_RS13730 Genome accession   NZ_CP034441
Coordinates   2616695..2617192 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain PMB 81-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2611695..2622192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN89_RS13690 - 2611739..2612725 (+) 987 WP_000161314.1 ROK family glucokinase -
  EJN89_RS13695 - 2612725..2613054 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EJN89_RS13700 - 2613051..2613674 (+) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  EJN89_RS13705 comGA 2613726..2614700 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EJN89_RS13710 comGB 2614672..2615742 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EJN89_RS13715 comGC 2615756..2616067 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EJN89_RS13720 comGD 2616045..2616491 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EJN89_RS13725 comGE 2616478..2616777 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  EJN89_RS13730 comGF 2616695..2617192 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  EJN89_RS13735 - 2617289..2617435 (+) 147 WP_001789879.1 hypothetical protein -
  EJN89_RS13740 - 2617425..2617949 (+) 525 WP_001015121.1 shikimate kinase -
  EJN89_RS13745 gcvT 2618108..2619199 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EJN89_RS13750 gcvPA 2619219..2620565 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EJN89_RS13755 gcvPB 2620558..2622030 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=331324 EJN89_RS13730 WP_001789864.1 2616695..2617192(+) (comGF) [Staphylococcus aureus strain PMB 81-4]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=331324 EJN89_RS13730 WP_001789864.1 2616695..2617192(+) (comGF) [Staphylococcus aureus strain PMB 81-4]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment