Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EJO50_RS12320 Genome accession   NZ_CP034433
Coordinates   2809542..2810915 (-) Length   457 a.a.
NCBI ID   WP_125974576.1    Uniprot ID   A0A3S8ZUR1
Organism   Iodobacter ciconiae strain H11R3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2804542..2815915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJO50_RS12285 (EJO50_12285) - 2804685..2805293 (-) 609 WP_125974562.1 flavin prenyltransferase UbiX -
  EJO50_RS12290 (EJO50_12290) - 2805834..2806358 (-) 525 WP_125974564.1 Gx transporter family protein -
  EJO50_RS12295 (EJO50_12295) - 2806333..2806692 (-) 360 WP_206434386.1 NusG domain II-containing protein -
  EJO50_RS12300 (EJO50_12300) rapZ 2806766..2807614 (-) 849 WP_125974568.1 RNase adapter RapZ -
  EJO50_RS12305 (EJO50_12305) hprK 2807687..2808631 (-) 945 WP_125974570.1 HPr(Ser) kinase/phosphatase -
  EJO50_RS12310 (EJO50_12310) ptsN 2808612..2809085 (-) 474 WP_125974572.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EJO50_RS12315 (EJO50_12315) hpf 2809191..2809517 (-) 327 WP_125974574.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EJO50_RS12320 (EJO50_12320) rpoN/rpoN1 2809542..2810915 (-) 1374 WP_125974576.1 RNA polymerase factor sigma-54 Machinery gene
  EJO50_RS12325 (EJO50_12325) lptB 2810916..2811641 (-) 726 WP_125974578.1 LPS export ABC transporter ATP-binding protein -
  EJO50_RS12330 (EJO50_12330) lptA 2811638..2812141 (-) 504 WP_125974580.1 lipopolysaccharide transport periplasmic protein LptA -
  EJO50_RS12335 (EJO50_12335) lptC 2812119..2812697 (-) 579 WP_125974582.1 LPS export ABC transporter periplasmic protein LptC -
  EJO50_RS12340 (EJO50_12340) - 2812697..2813212 (-) 516 WP_125974584.1 HAD family hydrolase -
  EJO50_RS12345 (EJO50_12345) - 2813411..2814382 (-) 972 WP_125974586.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 51742.73 Da        Isoelectric Point: 4.9142

>NTDB_id=331222 EJO50_RS12320 WP_125974576.1 2809542..2810915(-) (rpoN/rpoN1) [Iodobacter ciconiae strain H11R3]
MKQTLQLRLSQQLNLTPQLQQAIKLLQLSTLDFQQELEQYLSENPLLERGEEAAAGEAEQPESQWEIGEDDGLRWEGSSH
SGSDDDEDFDPASNIARDLDLREYLLQQAGLLPLSHRDKSLLLMLIEALDDDGFLSQSLEEVFSQLPEELIQDYELEPDD
LRIALIRLQHLEPLGVGARDTADCLCLQLRQKPDSAARELALKIAHNSLDLLAGRDYTRLKRTFKCEDGELKAAQELIVS
LNPRPSAQWSAASPRYVSPDVIVQKVRGAWRVRLHDASMPKLRVNRLYADILQKGDGGHLARELQEARWLVKSVQQRFDT
ILRVATAIVERQRAFFEHGEVAMRPLVLRDIAEELDLHESTISRVTTQKYMLTPRGLYEFKFFFGSHVDTDAGGECSATA
IKALIKQMVQQEDKRKPLSDSVLAEALSAQGIVVARRTVAKYREALQIYPVSQRKSL

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=331222 EJO50_RS12320 WP_125974576.1 2809542..2810915(-) (rpoN/rpoN1) [Iodobacter ciconiae strain H11R3]
ATGAAACAAACTCTGCAGCTGCGGCTATCCCAGCAACTTAATCTTACTCCCCAACTACAGCAGGCGATCAAGCTGCTGCA
GCTTTCTACCCTTGATTTTCAGCAAGAGCTTGAACAGTATCTGAGTGAAAATCCGCTATTGGAGCGGGGAGAGGAAGCTG
CGGCTGGTGAAGCTGAGCAGCCGGAGTCCCAGTGGGAAATAGGAGAGGACGACGGCTTGCGCTGGGAGGGCTCCAGCCAT
AGCGGCTCGGATGATGATGAAGATTTTGATCCTGCCAGCAATATTGCCCGTGATTTGGATTTGCGTGAATATCTGTTACA
GCAGGCGGGACTGCTGCCCCTCTCCCATAGGGATAAATCGTTATTGCTGATGTTGATAGAGGCGTTGGATGATGACGGTT
TTCTTTCTCAATCCTTAGAGGAGGTTTTTTCTCAGCTGCCAGAGGAACTAATTCAGGATTATGAGCTCGAACCTGATGAC
TTAAGGATTGCTTTAATTCGCTTACAGCACCTGGAGCCGCTTGGGGTGGGCGCAAGGGATACGGCAGATTGTTTATGCTT
ACAACTGCGCCAAAAACCCGATAGTGCCGCAAGAGAATTAGCATTAAAAATTGCCCATAATTCGCTCGATTTATTAGCCG
GACGGGACTACACTAGGCTTAAACGAACCTTCAAATGTGAAGATGGTGAGTTAAAAGCTGCACAAGAATTGATTGTAAGT
TTAAATCCAAGACCAAGTGCTCAATGGTCTGCAGCGTCACCCAGATATGTAAGCCCGGATGTGATTGTGCAAAAGGTAAG
AGGTGCCTGGCGTGTCAGATTACACGATGCCTCCATGCCAAAATTACGGGTAAACCGCCTTTATGCAGATATCTTACAAA
AAGGTGATGGCGGACATCTGGCGCGGGAGTTACAGGAAGCCCGCTGGCTGGTTAAGAGTGTACAGCAGCGTTTCGACACT
ATTTTACGAGTGGCCACGGCAATTGTAGAGCGGCAGCGTGCGTTTTTTGAACATGGTGAAGTGGCAATGCGCCCGTTGGT
ACTCAGGGATATTGCTGAAGAATTAGATTTACATGAGTCCACTATTTCCAGGGTGACCACGCAAAAATATATGCTTACGC
CGCGTGGCCTGTATGAATTCAAGTTTTTCTTCGGTAGCCATGTTGATACGGATGCCGGGGGAGAGTGTTCTGCTACAGCG
ATCAAAGCATTGATCAAGCAGATGGTGCAGCAAGAAGATAAGAGAAAACCTTTAAGTGATAGTGTGTTGGCAGAAGCTTT
GTCTGCTCAGGGTATTGTGGTAGCACGTCGTACAGTTGCCAAATACCGCGAGGCATTGCAGATTTATCCAGTAAGTCAGC
GCAAAAGTTTGTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(94-288)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(300-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.381

100

0.578

Multiple sequence alignment