Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EGY20_RS26020 Genome accession   NZ_CP033750
Coordinates   2479830..2481335 (+) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain FDAARGOS_547     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474830..2486335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY20_RS25995 (EGY20_25890) kdsD 2476081..2477064 (+) 984 WP_123790853.1 arabinose 5-phosphate isomerase KdsD -
  EGY20_RS26000 (EGY20_25895) - 2477064..2477600 (+) 537 WP_006398259.1 KdsC family phosphatase -
  EGY20_RS26005 (EGY20_25900) lptC 2477604..2478218 (+) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  EGY20_RS26010 (EGY20_25905) lptA 2478239..2478904 (+) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  EGY20_RS26015 (EGY20_25910) lptB 2478901..2479677 (+) 777 WP_123790854.1 LPS export ABC transporter ATP-binding protein -
  EGY20_RS26020 (EGY20_25915) rpoN/rpoN1 2479830..2481335 (+) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  EGY20_RS26025 (EGY20_25920) hpf 2481494..2481853 (+) 360 WP_006398254.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EGY20_RS26030 (EGY20_25925) - 2482048..2482560 (+) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  EGY20_RS26035 (EGY20_25930) hprK 2482689..2483657 (+) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  EGY20_RS26040 (EGY20_25935) rapZ 2483736..2484644 (+) 909 WP_006409809.1 RNase adapter RapZ -
  EGY20_RS26045 (EGY20_25940) - 2484672..2485307 (+) 636 WP_006398250.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=325416 EGY20_RS26020 WP_006398255.1 2479830..2481335(+) (rpoN/rpoN1) [Burkholderia multivorans strain FDAARGOS_547]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=325416 EGY20_RS26020 WP_006398255.1 2479830..2481335(+) (rpoN/rpoN1) [Burkholderia multivorans strain FDAARGOS_547]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTATGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAATTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCACTGCGCGACGCACACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGTGGGCCAGGGCTGGCAGGCCGAGCTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment