Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EGY16_RS00655 Genome accession   NZ_CP033703
Coordinates   125973..127481 (-) Length   502 a.a.
NCBI ID   WP_076859388.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain FDAARGOS_593     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120973..132481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY16_RS00625 (EGY16_00620) - 121987..122619 (-) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -
  EGY16_RS00630 (EGY16_00625) rapZ 122646..123539 (-) 894 WP_004195227.1 RNase adapter RapZ -
  EGY16_RS00635 (EGY16_00630) hprK 123625..124593 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  EGY16_RS00640 (EGY16_00635) ptsN 124722..125231 (-) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EGY16_RS00645 (EGY16_00640) hpf 125464..125823 (-) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EGY16_RS00655 (EGY16_00650) rpoN/rpoN1 125973..127481 (-) 1509 WP_076859388.1 RNA polymerase factor sigma-54 Machinery gene
  EGY16_RS00660 (EGY16_00655) lptB 127650..128426 (-) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  EGY16_RS00665 (EGY16_00660) lptA 128423..129088 (-) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  EGY16_RS00670 (EGY16_00665) lptC 129107..129721 (-) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  EGY16_RS00675 (EGY16_00670) - 129727..130266 (-) 540 WP_004200146.1 HAD family hydrolase -
  EGY16_RS00680 (EGY16_00675) kdsD 130266..131249 (-) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54949.10 Da        Isoelectric Point: 4.8740

>NTDB_id=324950 EGY16_RS00655 WP_076859388.1 125973..127481(-) (rpoN/rpoN1) [Burkholderia pseudomallei strain FDAARGOS_593]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETTGVDEYDSYSADGGDASQWNLDDFGRSPTASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=324950 EGY16_RS00655 WP_076859388.1 125973..127481(-) (rpoN/rpoN1) [Burkholderia pseudomallei strain FDAARGOS_593]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCACCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCACCGCGTCCGACGACGATGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment