Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EGV95_RS27625 Genome accession   NZ_CP033684
Coordinates   5725714..5726166 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H26027     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5720714..5731166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGV95_RS27605 (EGV95_27515) ampD 5721420..5721986 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EGV95_RS27610 (EGV95_27520) - 5722137..5724392 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  EGV95_RS27615 (EGV95_27525) nadC 5724678..5725526 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGV95_RS27625 (EGV95_27535) pilA 5725714..5726166 (-) 453 WP_024947914.1 pilin Machinery gene
  EGV95_RS27630 (EGV95_27540) pilB 5726395..5728095 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGV95_RS27635 (EGV95_27545) pilC 5728099..5729319 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  EGV95_RS27640 (EGV95_27550) pilD 5729323..5730195 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EGV95_RS27645 (EGV95_27555) coaE 5730192..5730803 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  EGV95_RS27650 (EGV95_27560) yacG 5730800..5731000 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=324736 EGV95_RS27625 WP_024947914.1 5725714..5726166(-) (pilA) [Pseudomonas aeruginosa strain H26027]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=324736 EGV95_RS27625 WP_024947914.1 5725714..5726166(-) (pilA) [Pseudomonas aeruginosa strain H26027]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387

Multiple sequence alignment