Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EFO25_RS29565 Genome accession   NZ_CP033645
Coordinates   3555116..3556627 (+) Length   503 a.a.
NCBI ID   WP_015877038.1    Uniprot ID   A0AAQ0BTD2
Organism   Burkholderia glumae strain YH43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3550116..3561627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFO25_RS29540 kdsD 3551334..3552317 (+) 984 WP_015877033.1 arabinose 5-phosphate isomerase KdsD -
  EFO25_RS29545 - 3552317..3552856 (+) 540 WP_015877034.1 KdsC family phosphatase -
  EFO25_RS29550 lptC 3552860..3553474 (+) 615 WP_015877035.1 LPS export ABC transporter periplasmic protein LptC -
  EFO25_RS29555 lptA 3553493..3554164 (+) 672 WP_015877036.1 lipopolysaccharide transport periplasmic protein LptA -
  EFO25_RS29560 lptB 3554161..3554937 (+) 777 WP_015877037.1 LPS export ABC transporter ATP-binding protein -
  EFO25_RS29565 rpoN/rpoN1 3555116..3556627 (+) 1512 WP_015877038.1 RNA polymerase factor sigma-54 Machinery gene
  EFO25_RS29570 hpf 3556779..3557138 (+) 360 WP_015877039.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EFO25_RS29575 ptsN 3557355..3557867 (+) 513 WP_015877040.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EFO25_RS29580 hprK 3558002..3558970 (+) 969 WP_015877041.1 HPr(Ser) kinase/phosphatase -
  EFO25_RS29585 rapZ 3559045..3559938 (+) 894 WP_015877042.1 RNase adapter RapZ -
  EFO25_RS29590 - 3559969..3560604 (+) 636 WP_015877043.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55057.11 Da        Isoelectric Point: 4.8365

>NTDB_id=324647 EFO25_RS29565 WP_015877038.1 3555116..3556627(+) (rpoN/rpoN1) [Burkholderia glumae strain YH43]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSSAPEPMQ
GAPVGGDAPAGERSERDDGSGGDDYDSYSGSDGDASQWNLDDYGRTSGASDDDDLPPLQVHETSTSLRDHLSAQLRVTQA
SPRDRALITFLIESLDDDGYLGATLDEVRADLPEELEVELDELAAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTR
TLALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKSGQNWLAELNPEV
VPKLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=324647 EFO25_RS29565 WP_015877038.1 3555116..3556627(+) (rpoN/rpoN1) [Burkholderia glumae strain YH43]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCACCTCGCGCTGACTCCCCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAAAACGACGACGAGTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGACGCCGCCGTCCTCGGCGCCCGAGCCGATGCAG
GGTGCGCCCGTCGGCGGCGACGCGCCGGCCGGCGAGCGCAGCGAGCGCGACGACGGCTCGGGCGGCGACGACTACGACAG
CTACTCCGGCAGCGACGGCGATGCGAGCCAGTGGAACCTCGACGACTACGGCCGCACTTCGGGCGCCTCCGACGACGACG
ATCTCCCGCCGCTGCAGGTCCACGAGACCTCGACTTCGCTGCGCGACCACCTGTCGGCACAGCTGCGCGTCACCCAGGCA
AGCCCGCGCGACCGCGCGCTGATCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTGGGCGCGACGCTCGACGA
AGTCCGCGCCGACCTGCCCGAGGAGCTCGAGGTCGAGCTCGACGAGCTGGCCGCCGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCCGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGCCTGCAGCTGCTGCGACTCGATCCGTCCCCCACGCGC
ACGCTCGCGCTCGACATCGTTTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAA
GGCGAGCGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCCGCCTACGGCAAGG
CCGAGGCCGACTACGTGGTGCCCGACATCATGGTGAAGAAGAGCGGCCAGAACTGGCTCGCGGAGCTGAATCCGGAAGTC
GTGCCCAAGCTGCGCATCAACCACCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCCGGCGCCGGCTCGCTGAA
GCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGATTCGAGACCATCCTGCGCGTCGCGCAAGCAA
TCGTCGAGCGCCAGAAGAACTTTTTTGCACATGGCGAAATAGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAA
GTACTTCTTCGGGTCGCATGTCTCGACGGATACCGGCGGCGCCGCCTCATCCACGGCGATCCGCGCCCTCATCAAGCAAC
TGATAGGAGCCGAAGACCCGAAAACTCCTTTATCGGACAGCCGAATCGCCGAGCTGCTGGCGGAACAGGGATTCGTGGTC
GCGCGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCAGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.992

100

0.68

Multiple sequence alignment