Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D2B33_RS06485 Genome accession   NZ_CP033198
Coordinates   1235537..1236166 (+) Length   209 a.a.
NCBI ID   WP_020452274.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain FA6     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230537..1241166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2B33_RS06450 (D2B33_06490) aroE 1231180..1232016 (+) 837 WP_020452280.1 shikimate dehydrogenase -
  D2B33_RS06455 (D2B33_06495) yhbY 1232010..1232300 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  D2B33_RS06460 (D2B33_06500) - 1232319..1232888 (+) 570 WP_020452279.1 nicotinate-nucleotide adenylyltransferase -
  D2B33_RS06465 (D2B33_06505) yqeK 1232878..1233441 (+) 564 WP_020452278.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D2B33_RS06470 (D2B33_06510) rsfS 1233455..1233811 (+) 357 WP_020452277.1 ribosome silencing factor -
  D2B33_RS06475 (D2B33_06515) - 1233808..1234563 (+) 756 WP_020452276.1 class I SAM-dependent methyltransferase -
  D2B33_RS06480 (D2B33_06520) comER 1234633..1235454 (-) 822 WP_020452275.1 late competence protein ComER -
  D2B33_RS06485 (D2B33_06525) comEA 1235537..1236166 (+) 630 WP_020452274.1 helix-hairpin-helix domain-containing protein Machinery gene
  D2B33_RS06490 (D2B33_06530) - 1236235..1236804 (+) 570 WP_020452273.1 ComE operon protein 2 -
  D2B33_RS06495 (D2B33_06535) - 1236809..1239196 (+) 2388 WP_041817260.1 DNA internalization-related competence protein ComEC/Rec2 -
  D2B33_RS06500 (D2B33_06540) - 1239221..1239352 (-) 132 WP_003183686.1 YqzM family protein -
  D2B33_RS06505 (D2B33_06545) holA 1239582..1240607 (+) 1026 WP_020452271.1 DNA polymerase III subunit delta -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22592.49 Da        Isoelectric Point: 4.6439

>NTDB_id=322432 D2B33_RS06485 WP_020452274.1 1235537..1236166(+) (comEA) [Bacillus paralicheniformis strain FA6]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSDISIPEEASAQTFDKKEEVKLKKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSGDIVNINTASSEELQT
IPGIGPSKAEAVIEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=322432 D2B33_RS06485 WP_020452274.1 1235537..1236166(+) (comEA) [Bacillus paralicheniformis strain FA6]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGAGTGGCACTCGTCTTGATCATCAGCGCGGCCTTCAT
GCTGTTAAGCGGAAAGCGCGAAACTTCATCCGACATTTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAAGTGAAGCTGAAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
AATTAATCTGGCGGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAGGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAATCGAATACCGTGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(146-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.804

100

0.541
  comEA Staphylococcus aureus MW2

35.909

100

0.378
  comEA Staphylococcus aureus N315

35.455

100

0.373

Multiple sequence alignment