Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FC605_RS13035 Genome accession   NZ_CP040528
Coordinates   2527935..2528345 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain PR10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2522935..2533345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FC605_RS13005 (FC605_13005) - 2523022..2523162 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  FC605_RS13010 (FC605_13010) yqeF 2523468..2524199 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  FC605_RS13015 (FC605_13015) cwlH 2524451..2525203 (-) 753 WP_080529562.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  FC605_RS13020 (FC605_13020) yqeD 2525390..2526016 (+) 627 WP_080529563.1 TVP38/TMEM64 family protein -
  FC605_RS13025 (FC605_13025) gnd 2526035..2526928 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  FC605_RS13030 (FC605_13030) yqeB 2527180..2527902 (+) 723 WP_080529564.1 hypothetical protein -
  FC605_RS13035 (FC605_13035) nucA/comI 2527935..2528345 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  FC605_RS13040 (FC605_13040) - 2528541..2528891 (+) 351 Protein_2512 sigma-70 family RNA polymerase sigma factor -
  FC605_RS13045 (FC605_13045) - 2529031..2529501 (+) 471 WP_080529565.1 MarR family transcriptional regulator -
  FC605_RS13050 (FC605_13050) - 2529653..2531065 (+) 1413 WP_080529566.1 MDR family MFS transporter -
  FC605_RS13055 (FC605_13055) spoIVCA 2531252..2532712 (-) 1461 WP_250622053.1 site-specific DNA recombinase SpoIVCA -
  FC605_RS21345 (FC605_13060) - 2532670..2532848 (-) 179 Protein_2516 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=321941 FC605_RS13035 WP_009967785.1 2527935..2528345(-) (nucA/comI) [Bacillus subtilis strain PR10]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=321941 FC605_RS13035 WP_009967785.1 2527935..2528345(-) (nucA/comI) [Bacillus subtilis strain PR10]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529