Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   D8682_RS17990 Genome accession   NZ_CP033076
Coordinates   3529304..3530737 (-) Length   477 a.a.
NCBI ID   WP_064543108.1    Uniprot ID   -
Organism   Buttiauxella sp. 3AFRM03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3524304..3535737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8682_RS17960 (D8682_17960) elbB 3525734..3526387 (+) 654 WP_121814813.1 isoprenoid biosynthesis glyoxalase ElbB -
  D8682_RS17965 (D8682_17965) mtgA 3526384..3527112 (+) 729 WP_121814814.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  D8682_RS17970 (D8682_17970) npr 3527118..3527390 (-) 273 WP_064543100.1 PTS phosphocarrier protein NPr -
  D8682_RS17975 (D8682_17975) rapZ 3527387..3528241 (-) 855 WP_120064597.1 RNase adapter RapZ -
  D8682_RS17980 (D8682_17980) ptsN 3528295..3528786 (-) 492 WP_064543104.1 PTS IIA-like nitrogen regulatory protein PtsN -
  D8682_RS17985 (D8682_17985) hpf 3528994..3529281 (-) 288 WP_064543106.1 ribosome hibernation promoting factor -
  D8682_RS17990 (D8682_17990) rpoN/rpoN1 3529304..3530737 (-) 1434 WP_064543108.1 RNA polymerase factor sigma-54 Machinery gene
  D8682_RS17995 (D8682_17995) lptB 3530911..3531636 (-) 726 WP_064543110.1 LPS export ABC transporter ATP-binding protein -
  D8682_RS18000 (D8682_18000) lptA 3531643..3532206 (-) 564 WP_064543111.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  D8682_RS18005 (D8682_18005) lptC 3532175..3532750 (-) 576 WP_064543113.1 LPS export ABC transporter periplasmic protein LptC -
  D8682_RS18010 (D8682_18010) kdsC 3532747..3533313 (-) 567 WP_064543115.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  D8682_RS18015 (D8682_18015) kdsD 3533330..3534316 (-) 987 WP_064543117.1 arabinose-5-phosphate isomerase KdsD -
  D8682_RS18020 (D8682_18020) - 3534332..3535309 (-) 978 WP_064543119.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53900.88 Da        Isoelectric Point: 4.3939

>NTDB_id=321192 D8682_RS17990 WP_064543108.1 3529304..3530737(-) (rpoN/rpoN1) [Buttiauxella sp. 3AFRM03]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVETQEVRENEALDTREALEQKEMPD
ELPLDTSWDEIYTAGTPSGTGSDYIDDELPVYQGETTQSLQDYLMWQVDLTPFSDTDTAIATSIVDAVDEMGYLTVSLEE
ILESMGDDEITLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFARETPWLTEASLIVSDHLDLLANHDFRSLMRVTR
LKEEVLKEAMCLIQSLDPRPGQSIQTGEPEYVIPDVLVKKTQGHWTVELNADSLPRLKINQQYAAMGTSARNDSDSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQGFFEHGEEHMKPMVLADIAQVVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=321192 D8682_RS17990 WP_064543108.1 3529304..3530737(-) (rpoN/rpoN1) [Buttiauxella sp. 3AFRM03]
ATGAAGCAAGGTTTGCAATTACGGCTTAGCCAACAACTTGCTATGACTCCCCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTTTCGACGTTAGAGCTACAGCAAGAACTTCAGCAAGCACTGGAAAGTAACCCACTGCTTGAGCAAACCGATCTGCATG
AAGAAGTTGAAACTCAGGAAGTACGTGAAAACGAAGCATTAGATACCCGTGAGGCGCTTGAGCAAAAAGAAATGCCTGAC
GAGTTACCGCTGGATACCAGTTGGGACGAAATCTACACCGCAGGCACTCCATCAGGAACCGGAAGCGATTATATCGACGA
CGAATTGCCGGTTTATCAGGGGGAAACGACCCAGTCTCTTCAGGATTATCTGATGTGGCAGGTTGATCTCACCCCGTTTT
CAGATACTGACACAGCAATTGCGACCTCTATTGTCGATGCCGTTGACGAAATGGGTTATCTCACAGTTTCTCTGGAAGAA
ATTCTGGAAAGCATGGGTGATGACGAAATCACCCTCGACGAAGTGGAAGCCGTACTCAAACGCGTTCAACGCTTCGATCC
TGTGGGTGTTGCCGCACGTGATCTACGCGATTGCCTGCTCATACAGCTTTCACAGTTCGCTCGCGAAACGCCATGGTTAA
CCGAAGCCAGCTTGATTGTCAGCGATCACCTGGATCTGCTCGCTAACCATGACTTCCGCAGCCTGATGCGCGTCACCCGT
CTGAAAGAAGAGGTGCTGAAAGAGGCAATGTGCCTGATTCAGTCTCTTGACCCACGCCCAGGGCAATCCATTCAGACCGG
TGAACCGGAATATGTCATACCGGATGTTCTGGTGAAAAAAACGCAAGGTCACTGGACTGTAGAACTCAATGCCGACAGCC
TCCCTCGTCTGAAAATAAACCAGCAGTATGCTGCAATGGGCACCAGTGCCCGCAATGACAGCGACTCGCAATTTATTCGC
AGTAATCTGCAAGAAGCAAAGTGGCTGATTAAGAGCCTGGAAAGCCGGAACGACACGCTGCTGCGCGTCAGTCGCTGTAT
CGTTGAACAGCAACAAGGCTTTTTTGAACACGGCGAAGAGCACATGAAACCGATGGTGCTTGCTGATATCGCTCAGGTCG
TAGAAATGCATGAATCGACCATTTCCCGAGTGACCACGCAAAAATATTTACATAGCCCACGCGGCATTTTTGAATTGAAG
TATTTCTTCTCAAGCCACGTTAATACCGAAGGTGGAGGTGAAGCCTCCTCGACGGCAATTCGTGCATTAGTGAAGAAATT
AATTGCTGCGGAAAACCCTGCGAAACCACTGAGTGATAGCAAGTTAACGACTATGCTATCGGATCAGGGGATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATACCACCGTCAAATCAGCGTAAACAGTTGGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.525

100

0.491

Multiple sequence alignment