Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CFN54_RS06915 Genome accession   NZ_CP032635
Coordinates   1378982..1379494 (-) Length   170 a.a.
NCBI ID   WP_035145013.1    Uniprot ID   A0A9N7J0G1
Organism   Latilactobacillus sakei strain ZFM225     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1373982..1384494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN54_RS06885 (CFN54_06885) - 1374908..1375273 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  CFN54_RS06895 (CFN54_06895) - 1375816..1376274 (-) 459 WP_035145008.1 hypothetical protein -
  CFN54_RS06900 (CFN54_06900) - 1376329..1377525 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  CFN54_RS06905 (CFN54_06905) - 1377547..1378557 (-) 1011 WP_016265372.1 class I SAM-dependent methyltransferase -
  CFN54_RS06910 (CFN54_06910) - 1378681..1378947 (-) 267 WP_016265373.1 hypothetical protein -
  CFN54_RS06915 (CFN54_06915) comGF 1378982..1379494 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFN54_RS06920 (CFN54_06920) comGE 1379469..1379774 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  CFN54_RS06925 (CFN54_06925) comGD 1379761..1380165 (-) 405 WP_235596237.1 hypothetical protein Machinery gene
  CFN54_RS06930 (CFN54_06930) comGC 1380185..1380484 (-) 300 WP_025016383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CFN54_RS06935 (CFN54_06935) comGB 1380481..1381488 (-) 1008 WP_061827023.1 type II secretion system F family protein Machinery gene
  CFN54_RS06940 (CFN54_06940) comGA 1381481..1382371 (-) 891 WP_025016380.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN54_RS06945 (CFN54_06945) - 1382488..1383219 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CFN54_RS06950 (CFN54_06950) - 1383310..1383810 (-) 501 WP_016265379.1 VanZ family protein -
  CFN54_RS06955 (CFN54_06955) - 1383889..1384281 (+) 393 WP_231952369.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19689.58 Da        Isoelectric Point: 6.9899

>NTDB_id=317434 CFN54_RS06915 WP_035145013.1 1378982..1379494(-) (comGF) [Latilactobacillus sakei strain ZFM225]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLNGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=317434 CFN54_RS06915 WP_035145013.1 1378982..1379494(-) (comGF) [Latilactobacillus sakei strain ZFM225]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTCACGCAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAAATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment