Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1106_RS06660 Genome accession   NZ_CP031861
Coordinates   1458967..1459515 (-) Length   182 a.a.
NCBI ID   WP_237593742.1    Uniprot ID   A0A9Q4H8T0
Organism   Actinobacillus pleuropneumoniae strain N 273     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1453967..1464515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1106_RS06645 (D1106_06690) - 1454449..1454970 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  D1106_RS06650 (D1106_06695) - 1455075..1456361 (+) 1287 WP_237593740.1 NAD(P)/FAD-dependent oxidoreductase -
  D1106_RS06655 (D1106_06700) - 1456553..1458892 (-) 2340 WP_237593741.1 Tex family protein -
  D1106_RS06660 (D1106_06705) pilF 1458967..1459515 (-) 549 WP_237593742.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1106_RS06665 (D1106_06710) - 1459569..1460750 (-) 1182 WP_237593743.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1106_RS06690 (D1106_06735) gltX 1461654..1463093 (+) 1440 WP_237592596.1 glutamate--tRNA ligase -
  D1106_RS06700 (D1106_06745) yefM 1463431..1463682 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1106_RS06705 (D1106_06750) - 1463679..1463939 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  D1106_RS06710 (D1106_06755) - 1464098..1464265 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20628.24 Da        Isoelectric Point: 6.9477

>NTDB_id=312054 D1106_RS06660 WP_237593742.1 1458967..1459515(-) (pilF) [Actinobacillus pleuropneumoniae strain N 273]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSNNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=312054 D1106_RS06660 WP_237593742.1 1458967..1459515(-) (pilF) [Actinobacillus pleuropneumoniae strain N 273]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACCTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGTTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAGCTTAGTAATAACCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.553

100

0.698
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445

Multiple sequence alignment