Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   DWF74_RS18225 Genome accession   NZ_CP031396
Coordinates   4025910..4028129 (-) Length   739 a.a.
NCBI ID   WP_115581438.1    Uniprot ID   -
Organism   Pseudomonas protegens strain FD6     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4020910..4033129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWF74_RS18205 (DWF74_18205) hslU 4021129..4022466 (-) 1338 WP_102862794.1 ATP-dependent protease ATPase subunit HslU -
  DWF74_RS18210 (DWF74_18210) hslV 4022524..4023054 (-) 531 WP_003186641.1 ATP-dependent protease subunit HslV -
  DWF74_RS18215 (DWF74_18215) - 4023253..4023960 (-) 708 WP_016962745.1 SPOR domain-containing protein -
  DWF74_RS18220 (DWF74_18220) argS 4023962..4025698 (-) 1737 WP_102882089.1 arginine--tRNA ligase -
  DWF74_RS18225 (DWF74_18225) priA 4025910..4028129 (-) 2220 WP_115581438.1 primosomal protein N' Machinery gene
  DWF74_RS18230 (DWF74_18230) rpmE 4028429..4028650 (+) 222 WP_011058815.1 50S ribosomal protein L31 -
  DWF74_RS18235 (DWF74_18235) - 4028692..4029471 (+) 780 WP_115581439.1 thermonuclease family protein -
  DWF74_RS18240 (DWF74_18240) - 4029659..4030927 (+) 1269 WP_102862798.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82080.62 Da        Isoelectric Point: 8.5521

>NTDB_id=306546 DWF74_RS18225 WP_115581438.1 4025910..4028129(-) (priA) [Pseudomonas protegens strain FD6]
MPDAILRLALPSPLRRLFDYRAPAGVLRAQLHPGMRLRVPFGRREMIGILVEVADHSEVPAEKLKPALALLDPTPPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGELAEARQERFWSIAPGARLDDPRIARAPRQREALATLAQHPHGVPHALL
SKLMLSKDSLDLLLAKDLVQVEVRKHAPGVRHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRSGELRCHHCGHVERVPRHC
PQCGKVDLRPVGAGTERAEERLGILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGHPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACGEAERLLTEQNLGGIELLGPVPAPMERRAGRYRAQLLLQANARAPLHRLLSNWLLLLEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=306546 DWF74_RS18225 WP_115581438.1 4025910..4028129(-) (priA) [Pseudomonas protegens strain FD6]
GTGCCCGACGCCATCCTGCGCCTCGCCCTGCCTTCGCCCCTGCGCCGCTTGTTCGACTACCGTGCACCCGCCGGCGTGCT
GCGCGCCCAGCTGCATCCGGGCATGCGCCTGCGGGTGCCATTCGGCCGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
CGGATCACAGCGAAGTCCCGGCCGAGAAACTCAAGCCGGCGCTGGCCTTGCTGGACCCCACCCCGCCACTGCCACCCGCC
CTGTTCAAGCTGTGCCTGTGGACCGCCCAGTATTATCAGCACAGCCTGGGCGACACCCTGAGCTGGGCCTTGCCGGTGCT
GCTGCGCCAGGGAGAGCTGGCCGAGGCCCGCCAGGAGCGTTTCTGGTCGATTGCCCCTGGCGCCCGCCTTGATGACCCAC
GCATCGCCCGTGCACCGCGCCAGCGTGAAGCCCTGGCAACCCTGGCCCAGCATCCCCATGGCGTGCCCCATGCATTGCTG
AGCAAGCTGATGCTGAGCAAGGACAGCCTGGATCTGCTTCTGGCCAAGGACCTGGTGCAGGTGGAAGTACGCAAGCACGC
TCCAGGCGTGCGCCACGAACACTGGCTGGCCCAGCCCGAGTTGCCGCTCAACAGCGAGCAGCGCGCCGCCTACGAGGCCA
TCCGCGCCGGGTTCGACAGTTATCACGCCTTTCTCCTGGCTGGCGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAG
TTGATCCGCGAGACCCTAGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAGATCAACCTGGGGCCGCAAACCCTGGC
GCGCTTCGAGCAACGTTTCAACGCCCGCATCGCCCTGCTGCACTCGGCGGTCAATGATCGGGAACGCCTGGATGCCTGGC
TCGCCGCCCGGGATGGCGAAGCCGATATCATCATCGGCACCCGCTCGGCCCTGTTCACCCCGATGAAAAATCCCGGGCTG
ATCATCATCGACGAGGAGCACGATGGCTCCTATAAACAGCAGGAAGGCCTGCGCTACCACGCCCGGGACCTGGCACTGGT
GCGGGCACGCCAGGAAAACATCCCGATTGTCCTGGGCTCGGCCACCCCATCCCTGGAAAGCCTGCACAACGCCTACACCG
GTCGCTATGGCCTGCTGCGCCTGAACGAGCGGGCCGGTGGCGCCAAACAGCCACGCTTCCTGCGCCTGGATGTGAAGAGT
CGCCCCTTGGACAGCGGTATTTCAGGCCCCATGCAACAAGCCATCGGTCAGACCCTGGCCGCCGGGCAGCAAGTGCTGGT
GTTTCTCAACCGCCGGGGCTTCGCTCCGACCCTGCTCTGCCATGACTGCGGCTGGATGTCTGAATGTCAGCGCTGCGATG
CCCGGATGACCGTGCACCAGCGCTCCGGCGAGTTGCGCTGCCACCATTGTGGCCACGTCGAGCGCGTGCCCCGGCACTGC
CCGCAATGCGGCAAGGTCGACCTGCGCCCCGTGGGTGCCGGAACCGAGCGGGCGGAAGAACGCCTGGGAATACTCTTCCC
CGACTACCCGGTACTACGGGTGGACCGCGACAGCACCTCACGCAAGGACGCGATGAACCAACTGTTCGCCACCATCCAGA
AAGGTCATCCGTGCATCCTGGTGGGCACCCAGATGCTGGCCAAGGGGCACCACTTCCCCCGGGTCACCCTGGTATCGATC
CTCGATGCCGACGGCGGCCTGTTCTCCGGCGACTTCCGTGCCAGCGAGCGCATGGCGCAACTGATCGTCCAGGTGGCCGG
GCGCGCGGGACGCGCCGAAGAACCGGGCAAGGTGATCATCCAGACTCACCTCGCCGACCACCCGCTACTGGTGCAACTGA
CCGAACAGGGCTACTTCGCCTTTGCCGAGCAGGCCCTGAGCGAGCGCCGCGCCGCCGGCCTGCCACCCTTCGCCCATCTG
GCGCTGCTGCGGGCCGAAGCCCACAAGCCAGGCCAGGCCGAAGGTTTTCTGGACGAAGCCTGCGGCGAAGCCGAGCGCTT
GCTGACGGAACAGAACCTGGGCGGCATCGAACTGTTGGGCCCGGTGCCAGCGCCGATGGAGCGGCGCGCCGGACGTTACC
GTGCGCAATTGCTGCTGCAGGCCAACGCCCGGGCACCGCTGCACCGGCTACTCAGCAACTGGCTGCTGCTGCTGGAGCAG
ATGCCCAGCGGGCGCCAGGTGCGCTGGTCGCTGGATGTGGACCCGGTGGATTTGTATTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(207-367)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

48.253

100

0.486

Multiple sequence alignment