Detailed information NTG0030577

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: XFASM23_RS10230
• Genome accession: NC_010577
• Coordinates: 2275635..2277368 (+)
• Length: 577 a.a.
• NCBI ID: WP_004090374.1
• Uniprot ID: Q87AA1
• Organism: Xylella fastidiosa M23
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2270635..2282368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFASM23_RS10210 (XfasM23_2030)	pilC	2270954..2272156 (-)	1203	WP_011098309.1	type II secretion system F family protein	Machinery gene
XFASM23_RS10215 (XfasM23_2031)	-	2272597..2273043 (+)	447	WP_012382763.1	pilin	-
XFASM23_RS10220 (XfasM23_2032)	-	2273289..2273516 (-)	228	WP_011098310.1	hypothetical protein	-
XFASM23_RS10225 (XfasM23_2033)	-	2274352..2274792 (+)	441	WP_004090375.1	pilin	-
XFASM23_RS10230 (XfasM23_2034)	pilB	2275635..2277368 (+)	1734	WP_004090374.1	type IV-A pilus assembly ATPase PilB	Machinery gene
XFASM23_RS10235 (XfasM23_2035)	pilR	2277462..2278874 (-)	1413	WP_004090373.1	sigma-54 dependent transcriptional regulator	Regulator
XFASM23_RS10240 (XfasM23_2036)	-	2279060..2280670 (-)	1611	WP_004090372.1	ATP-binding protein	-
XFASM23_RS10245 (XfasM23_2037)	sucC	2280925..2282088 (+)	1164	WP_004090371.1	ADP-forming succinate--CoA ligase subunit beta	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62946.98 Da
• Isoelectric Point: 5.9144

>NTDB_id=30577 XFASM23_RS10230 WP_004090374.1 2275635..2277368(+) (pilB) [Xylella fastidiosa M23]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALD
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPAQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=30577 XFASM23_RS10230 WP_004090374.1 2275635..2277368(+) (pilB) [Xylella fastidiosa M23]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTACTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGAGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACTCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAT
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTCTCACTTTA
CACTGCGTTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGTGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACCCTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGCTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACTGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (200-467)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (48-152)

3D structure

• AlphaFold DB: Q87AA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	56.966	98.267	0.56
pilB	Acinetobacter baumannii D1279779	56.79	98.267	0.558
pilB	Legionella pneumophila strain ERS1305867	52.456	98.787	0.518
pilF	Neisseria gonorrhoeae MS11	50.264	98.614	0.496
pilB	Vibrio cholerae strain A1552	49.36	94.801	0.468
pilB	Vibrio parahaemolyticus RIMD 2210633	47.08	97.92	0.461
pilB	Vibrio campbellii strain DS40M4	46.303	98.44	0.456
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.993	92.894	0.362