Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   U12A_RS14050 Genome accession   NZ_CP035476
Coordinates   2243121..2244548 (+) Length   475 a.a.
NCBI ID   WP_000980556.1    Uniprot ID   -
Organism   Escherichia coli strain U12A     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2238121..2249548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U12A_RS14010 (U12A_02195) yeeW 2238305..2238499 (+) 195 WP_000988600.1 DUF5983 family protein -
  U12A_RS14015 - 2238545..2238625 (+) 81 Protein_2236 hypothetical protein -
  U12A_RS28535 - 2238914..2239087 (-) 174 WP_032082634.1 transposase domain-containing protein -
  U12A_RS28540 - 2239114..2239296 (+) 183 WP_001296208.1 EutP/PduV family microcompartment system protein -
  U12A_RS14030 (U12A_02196) tmaR 2239397..2239726 (-) 330 WP_000450409.1 DUF496 family protein -
  U12A_RS14035 (U12A_02197) yeeA 2239898..2240956 (-) 1059 WP_001200889.1 FUSC family protein -
  U12A_RS14040 (U12A_02198) sbmC 2241154..2241627 (-) 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
  U12A_RS14045 (U12A_02199) dacD 2241746..2242912 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  U12A_RS14050 (U12A_02200) sbcB 2243121..2244548 (+) 1428 WP_000980556.1 exodeoxyribonuclease I Machinery gene
  U12A_RS14065 (U12A_02201) tnpA 2244659..2245117 (-) 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
  U12A_RS14075 (U12A_02202) plaP 2245334..2246692 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  U12A_RS14080 yoeI 2246682..2246744 (-) 63 WP_010723108.1 membrane protein YoeI -
  U12A_RS14085 (U12A_02203) yeeY 2246959..2247888 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  U12A_RS14090 (U12A_02204) yeeZ 2247934..2248758 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  U12A_RS14095 (U12A_02205) yoeB 2248841..2249095 (-) 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  U12A_RS14100 (U12A_02206) yefM 2249092..2249343 (-) 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54437.70 Da        Isoelectric Point: 5.4700

>NTDB_id=303634 U12A_RS14050 WP_000980556.1 2243121..2244548(+) (sbcB) [Escherichia coli strain U12A]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=303634 U12A_RS14050 WP_000980556.1 2243121..2244548(+) (sbcB) [Escherichia coli strain U12A]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTAGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTTTGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACACCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583