Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   DN405_RS21735 Genome accession   NZ_CP031065
Coordinates   4201047..4203386 (-) Length   779 a.a.
NCBI ID   WP_006926735.1    Uniprot ID   -
Organism   Bacillus sp. SH8-8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4196047..4208386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN405_RS28215 - 4197294..4197470 (-) 177 WP_001242605.1 hypothetical protein -
  DN405_RS21720 (DN405_21705) - 4197636..4198076 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  DN405_RS21725 (DN405_21710) relA 4198093..4200276 (-) 2184 WP_001262795.1 GTP diphosphokinase -
  DN405_RS21730 (DN405_21715) - 4200487..4200999 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  DN405_RS21735 (DN405_21720) recJ 4201047..4203386 (-) 2340 WP_006926735.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  DN405_RS21740 (DN405_21725) - 4203488..4204381 (-) 894 WP_000409746.1 cation diffusion facilitator family transporter -
  DN405_RS21745 (DN405_21730) secDF 4204538..4206802 (-) 2265 WP_049107979.1 protein translocase subunit SecDF -
  DN405_RS21750 (DN405_21735) - 4207072..4207356 (-) 285 WP_000886979.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88136.70 Da        Isoelectric Point: 4.9019

>NTDB_id=303125 DN405_RS21735 WP_006926735.1 4201047..4203386(-) (recJ) [Bacillus sp. SH8-8]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=303125 DN405_RS21735 WP_006926735.1 4201047..4203386(-) (recJ) [Bacillus sp. SH8-8]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAACGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAACAAATATTAATATTTGGTGATTACGATGCAGACGGAGTAAGTAGTACAACTGTGTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTATATTCCAAATCGTTTTACAGAAGGGTATGGGCCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCGAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCTGATTTAGTGTCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAAAAATATTGGTTTAAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAACGC
CGGCTGTACACCTTTTATTATCAGATGATCCTGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTTGAAAATAACTTTCCGCCAGAAGAAAACAAAGT
GTTAGTACTTGCAAAAGAAGGTTGGAATCCGGGTGTAATTGGGATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACTATTGTATTATGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCGCGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTGCCACACTTTGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTAGAGGATGTAACACTAGCGGCAATTGAAGATATGCAAAAACTAGCCCCGTTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTGAAAGATGCAGAGTTAGAAAGTATTCGCGCGATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCCATCAATGAATGGAATAACTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGTGAG
CTATTTAAGGTAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCACTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.326

99.358

0.52

Multiple sequence alignment