Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SWOO_RS06805 Genome accession   NC_010506
Coordinates   1686201..1687313 (+) Length   370 a.a.
NCBI ID   WP_012323972.1    Uniprot ID   B1KIX8
Organism   Shewanella woodyi ATCC 51908     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681201..1692313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SWOO_RS06770 (Swoo_1327) rdgB 1681326..1681928 (-) 603 WP_012323965.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SWOO_RS06775 (Swoo_1328) - 1682058..1682492 (-) 435 WP_012323966.1 DUF4426 domain-containing protein -
  SWOO_RS06780 (Swoo_1329) yggU 1682556..1682843 (-) 288 WP_012323967.1 DUF167 family protein YggU -
  SWOO_RS06785 (Swoo_1330) - 1682843..1683391 (-) 549 WP_012323968.1 YggT family protein -
  SWOO_RS06790 (Swoo_1331) proC 1683449..1684267 (-) 819 WP_012323969.1 pyrroline-5-carboxylate reductase -
  SWOO_RS06795 (Swoo_1332) - 1684398..1685099 (-) 702 WP_041418012.1 YggS family pyridoxal phosphate-dependent enzyme -
  SWOO_RS06800 (Swoo_1333) pilT 1685136..1686173 (+) 1038 WP_012323971.1 type IV pilus twitching motility protein PilT Machinery gene
  SWOO_RS06805 (Swoo_1334) pilU 1686201..1687313 (+) 1113 WP_012323972.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SWOO_RS06810 (Swoo_1335) hemH 1687547..1688578 (+) 1032 WP_012323973.1 ferrochelatase -
  SWOO_RS06815 (Swoo_1336) ruvX 1688638..1689057 (-) 420 WP_012323974.1 Holliday junction resolvase RuvX -
  SWOO_RS06820 (Swoo_1337) - 1689125..1689685 (-) 561 WP_012323975.1 YqgE/AlgH family protein -
  SWOO_RS06825 (Swoo_1338) yciH 1689727..1690056 (-) 330 WP_012323976.1 stress response translation initiation inhibitor YciH -
  SWOO_RS06830 (Swoo_1339) - 1690245..1690559 (-) 315 WP_012323977.1 hypothetical protein -
  SWOO_RS06835 (Swoo_1340) - 1690729..1691298 (+) 570 WP_012323978.1 DUF4136 domain-containing protein -
  SWOO_RS06840 (Swoo_1341) - 1691479..1691931 (+) 453 WP_012323979.1 DUF924 family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40954.86 Da        Isoelectric Point: 5.7948

>NTDB_id=30312 SWOO_RS06805 WP_012323972.1 1686201..1687313(+) (pilU) [Shewanella woodyi ATCC 51908]
MDVRPFLKIMVERKASDLFITAGFPPSAKIDGELRPLGEHSFSAAQSLEFVESLMTDVQKEEFHNTRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETNIPDADDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNANARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALAFAETGHLCMATLHANNANQALDRI
MNLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGLGRRAAIEILINTPRVSSLIAKNELHSLKETMAKSNEQGMQTFDQA
LLNLYVEGEISYADALHHADSPNDLRLMIKLRSSETGGSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=30312 SWOO_RS06805 WP_012323972.1 1686201..1687313(+) (pilU) [Shewanella woodyi ATCC 51908]
ATGGACGTTCGTCCTTTTTTAAAAATTATGGTGGAACGCAAAGCGTCGGATCTGTTCATTACGGCAGGCTTCCCCCCCAG
TGCGAAGATAGATGGTGAGTTAAGACCTCTAGGTGAGCACTCTTTCTCTGCGGCGCAGTCATTGGAGTTTGTTGAGTCGT
TAATGACCGATGTGCAGAAGGAGGAGTTTCATAACACACGTGAGTGTAACTTCGCTTTTGCAGCAAAAGAGTTAGGAAGA
TTTCGTGTTAGTGCATTTTGGCAAAGGGAGTCTCCTGGTTGCGTGATGCGTCGTATCGAAACAAATATTCCTGATGCTGA
CGATCTCAAGCTTCCTCCCATCTTAAAAGATCTGGTGATGAGTAAGCGCGGTCTTATCATAATGGTAGGTGGTACAGGTA
CCGGTAAGTCTACCTCATTGGCTTCACTTGTTGGTTACCGTAATGCCAATGCTCGCGGGCATATTTTGACCATTGAGGAT
CCTGTCGAGTTTGTCCATGATCACCGCAAAAGTATCATTACTCAACGTGAGGTGGGCATAGATACAGAGTCGTTCGATGC
AGCCCTAAAAAGCTCACTACGACAGGCGCCCGATGTCATCTTAATTGGTGAGATCAGAACCCAAGAGACGATGGAATTTG
CGCTAGCCTTTGCTGAAACCGGTCATTTGTGTATGGCAACACTTCACGCCAATAATGCTAACCAAGCACTGGATCGTATT
ATGAACTTAGTGCCAGAGAGTAAGCATCAGCAGCTTTTGTTCGATCTCTCTTTGAACTTGCGTGGTATCGTGGCACAGCA
ACTTGTGCCTAAAGCGGATGGACTAGGGCGACGTGCGGCAATTGAGATCTTGATCAACACGCCAAGGGTGAGCAGTCTTA
TTGCTAAAAATGAACTTCACTCCCTTAAAGAGACAATGGCTAAGTCCAATGAGCAGGGGATGCAAACCTTCGATCAGGCG
CTGTTAAACCTCTATGTAGAAGGCGAGATTAGCTACGCCGATGCTCTGCACCATGCCGATTCACCAAACGATCTTCGTTT
GATGATAAAGCTGCGCAGTTCAGAAACTGGTGGCTCAGGCTTTATGGAGGGGGTGACGCTAGATCTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1KIX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.29

100

0.616
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilU Vibrio cholerae strain A1552

57.297

100

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.226

97.027

0.4
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment