Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   DN399_RS21160 Genome accession   NZ_CP031062
Coordinates   4096691..4097437 (-) Length   248 a.a.
NCBI ID   WP_000487015.1    Uniprot ID   A0A9W5E8B7
Organism   Bacillus sp. AR4-2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4091691..4102437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DN399_RS21145 (DN399_21140) dnaG 4092452..4094248 (-) 1797 WP_000528223.1 DNA primase -
  DN399_RS28160 - 4094330..4094473 (-) 144 WP_000479733.1 hypothetical protein -
  DN399_RS21150 (DN399_21145) - 4095109..4095921 (-) 813 WP_000368944.1 pyruvate, water dikinase regulatory protein -
  DN399_RS21155 (DN399_21150) - 4095951..4096583 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  DN399_RS21160 (DN399_21155) recO 4096691..4097437 (-) 747 WP_000487015.1 DNA repair protein RecO Machinery gene
  DN399_RS21165 (DN399_21160) - 4097471..4097614 (-) 144 WP_000883924.1 YqzL family protein -
  DN399_RS21170 (DN399_21165) era 4097791..4098696 (-) 906 WP_001080230.1 GTPase Era -
  DN399_RS21175 (DN399_21170) - 4098689..4099087 (-) 399 WP_001086285.1 cytidine deaminase -
  DN399_RS21180 (DN399_21175) - 4099187..4099540 (-) 354 WP_000715466.1 diacylglycerol kinase family protein -
  DN399_RS21185 (DN399_21180) ybeY 4099537..4100007 (-) 471 WP_000054684.1 rRNA maturation RNase YbeY -
  DN399_RS21190 (DN399_21185) - 4100004..4102148 (-) 2145 WP_000091509.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28634.21 Da        Isoelectric Point: 8.0566

>NTDB_id=303072 DN399_RS21160 WP_000487015.1 4096691..4097437(-) (recO) [Bacillus sp. AR4-2]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGSKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=303072 DN399_RS21160 WP_000487015.1 4096691..4097437(-) (recO) [Bacillus sp. AR4-2]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAAATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGTCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTAATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTAAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGCCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.061

99.597

0.548

Multiple sequence alignment