Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DDE72_RS14900 Genome accession   NZ_CP029034
Coordinates   3030388..3030825 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain LDO2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3025388..3035825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDE72_RS14885 (DDE72_14885) - 3028248..3028610 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  DDE72_RS14890 (DDE72_14890) hxlA 3028842..3029477 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  DDE72_RS14895 (DDE72_14895) hxlB 3029474..3030031 (+) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  DDE72_RS14900 (DDE72_14900) nucA/comI 3030388..3030825 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DDE72_RS14905 (DDE72_14905) nin/comJ 3030846..3031244 (+) 399 WP_032872809.1 competence protein ComJ Regulator
  DDE72_RS14910 (DDE72_14910) - 3031285..3031503 (-) 219 Protein_2896 family 1 glycosylhydrolase -
  DDE72_RS14920 (DDE72_14920) - 3031723..3032046 (-) 324 WP_032872805.1 YckD family protein -
  DDE72_RS14925 (DDE72_14925) - 3032119..3032553 (-) 435 WP_021494203.1 RDD family protein -
  DDE72_RS14930 (DDE72_14930) - 3032568..3033704 (-) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  DDE72_RS14935 (DDE72_14935) - 3033769..3034971 (-) 1203 WP_032872800.1 GTP-binding protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=289531 DDE72_RS14900 WP_003156588.1 3030388..3030825(+) (nucA/comI) [Bacillus velezensis strain LDO2]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=289531 DDE72_RS14900 WP_003156588.1 3030388..3030825(+) (nucA/comI) [Bacillus velezensis strain LDO2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment