Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGY23_RS03510 Genome accession   NZ_CP033832
Coordinates   751600..752223 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain FDAARGOS_505     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 746600..757223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY23_RS03490 (EGY23_03480) aroB 747195..748301 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  EGY23_RS03495 (EGY23_03485) aroK 748351..748869 (-) 519 WP_123789195.1 shikimate kinase AroK -
  EGY23_RS03500 (EGY23_03490) pilQ 748881..751025 (-) 2145 WP_023083249.1 type 4a pilus secretin PilQ Machinery gene
  EGY23_RS03505 (EGY23_03495) comL/pilP 751079..751603 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  EGY23_RS03510 (EGY23_03500) pilO 751600..752223 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGY23_RS03515 (EGY23_03505) pilN 752220..752816 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  EGY23_RS03520 (EGY23_03510) pilM 752816..753880 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  EGY23_RS03525 (EGY23_03515) - 754065..756536 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=284980 EGY23_RS03510 WP_003103268.1 751600..752223(-) (pilO) [Pseudomonas aeruginosa strain FDAARGOS_505]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=284980 EGY23_RS03510 WP_003103268.1 751600..752223(-) (pilO) [Pseudomonas aeruginosa strain FDAARGOS_505]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGTAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401