Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SPWS13_RS17835 Genome accession   NZ_CP028435
Coordinates   3946415..3947527 (+) Length   370 a.a.
NCBI ID   WP_011788675.1    Uniprot ID   A0A6N3J369
Organism   Shewanella putrefaciens strain WS13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3941415..3952527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPWS13_RS17800 (SPWS13_4195) rdgB 3941511..3942128 (-) 618 WP_025008694.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SPWS13_RS17805 (SPWS13_4196) - 3942239..3942673 (-) 435 WP_025008695.1 DUF4426 domain-containing protein -
  SPWS13_RS17810 (SPWS13_4197) yggU 3942751..3943041 (-) 291 WP_011919678.1 DUF167 family protein YggU -
  SPWS13_RS17815 (SPWS13_4198) - 3943041..3943589 (-) 549 WP_061783209.1 YggT family protein -
  SPWS13_RS17820 (SPWS13_4199) proC 3943636..3944454 (-) 819 WP_014611018.1 pyrroline-5-carboxylate reductase -
  SPWS13_RS17825 (SPWS13_4200) - 3944635..3945333 (-) 699 WP_128090277.1 YggS family pyridoxal phosphate-dependent enzyme -
  SPWS13_RS17830 - 3945369..3946405 (+) 1037 Protein_3449 type IV pilus twitching motility protein PilT -
  SPWS13_RS17835 (SPWS13_4203) pilU 3946415..3947527 (+) 1113 WP_011788675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SPWS13_RS17840 (SPWS13_4204) - 3947666..3948205 (+) 540 WP_025008699.1 glutathione peroxidase -
  SPWS13_RS17845 (SPWS13_4205) hemH 3948238..3949251 (+) 1014 WP_011788677.1 ferrochelatase -
  SPWS13_RS17850 (SPWS13_4206) ruvX 3949313..3949738 (-) 426 WP_025008700.1 Holliday junction resolvase RuvX -
  SPWS13_RS17855 (SPWS13_4207) - 3949770..3950333 (-) 564 WP_011788679.1 YqgE/AlgH family protein -
  SPWS13_RS17860 (SPWS13_4208) yciH 3950446..3950775 (-) 330 WP_014611015.1 stress response translation initiation inhibitor YciH -
  SPWS13_RS17865 (SPWS13_4209) - 3950940..3951254 (-) 315 WP_011919670.1 hypothetical protein -
  SPWS13_RS17870 (SPWS13_4210) - 3951433..3951993 (+) 561 WP_011788682.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41097.28 Da        Isoelectric Point: 6.3723

>NTDB_id=284816 SPWS13_RS17835 WP_011788675.1 3946415..3947527(+) (pilU) [Shewanella putrefaciens strain WS13]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=284816 SPWS13_RS17835 WP_011788675.1 3946415..3947527(+) (pilU) [Shewanella putrefaciens strain WS13]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGAGTCAGCGCTTTTTGGCAAAGGGAATCACCAGGATGTGTTATGCGCCGGATTGAGACCAAAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACAGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAACGTGAAGTAGGCATAGATACGGAGTCATTCGATGC
AGCATTAAAAAGCTCCCTTCGTCAGGCGCCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCCAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTGTTAATTAATACTCCAAGGGTGGCGAGTTTAA
TTGCCAAAAATGAACTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAGCAGGGGATGCAGACATTCGACCAAGCG
TTACTGAAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT
GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGTGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N3J369

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment