Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGD   Type   Machinery gene
Locus tag   D9Y32_RS21045 Genome accession   NZ_CP033218
Coordinates   4028356..4028793 (-) Length   145 a.a.
NCBI ID   WP_009327905.1    Uniprot ID   Q8VQ70
Organism   Bacillus licheniformis strain TCCC 11148     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 4023356..4033793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9Y32_RS20995 (D9Y32_21020) sinI 4023532..4023708 (+) 177 WP_003183444.1 anti-repressor SinI Regulator
  D9Y32_RS21000 (D9Y32_21025) sinR 4023742..4024077 (+) 336 WP_006637528.1 transcriptional regulator SinR Regulator
  D9Y32_RS21005 (D9Y32_21030) tasA 4024182..4024976 (-) 795 WP_142782737.1 biofilm matrix protein TasA -
  D9Y32_RS21010 (D9Y32_21035) sipW 4025050..4025634 (-) 585 WP_003183449.1 signal peptidase I SipW -
  D9Y32_RS21015 (D9Y32_21040) tapA 4025631..4026359 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  D9Y32_RS21020 (D9Y32_21045) - 4026636..4026956 (+) 321 WP_003183454.1 YqzG/YhdC family protein -
  D9Y32_RS21025 (D9Y32_21050) - 4026980..4027162 (-) 183 WP_003183456.1 YqzE family protein -
  D9Y32_RS21030 (D9Y32_21055) comGG 4027250..4027615 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  D9Y32_RS21035 (D9Y32_21060) comGF 4027628..4028116 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  D9Y32_RS21040 (D9Y32_21065) comGE 4028025..4028372 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  D9Y32_RS21045 (D9Y32_21070) comGD 4028356..4028793 (-) 438 WP_009327905.1 competence type IV pilus minor pilin ComGD Machinery gene
  D9Y32_RS21050 (D9Y32_21075) comGC 4028799..4029092 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  D9Y32_RS21055 (D9Y32_21080) comGB 4029106..4030140 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  D9Y32_RS21060 (D9Y32_21085) comGA 4030130..4031194 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  D9Y32_RS21065 (D9Y32_21090) - 4031351..4032190 (-) 840 WP_061576836.1 STAS domain-containing protein -
  D9Y32_RS21075 (D9Y32_21100) - 4032413..4032808 (-) 396 WP_142782738.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D9Y32_RS21080 (D9Y32_21105) - 4033017..4033262 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16249.07 Da        Isoelectric Point: 9.6739

>NTDB_id=282783 D9Y32_RS21045 WP_009327905.1 4028356..4028793(-) (comGD) [Bacillus licheniformis strain TCCC 11148]
MNQKGFTLLESLTVLMIGSIMFSLLFALLPPIYEKRIVQHFVSQLEEDLLYAQQTALVRHTTVKVQFDFAGCRYIMKHSD
EGSSPELVRTYSCNIAIKKSTLKPILTFNPSGVPNQGGKIVIDTQHASFILTIYLGSGKINVQKK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=282783 D9Y32_RS21045 WP_009327905.1 4028356..4028793(-) (comGD) [Bacillus licheniformis strain TCCC 11148]
ATGAATCAAAAAGGATTTACGCTTTTGGAAAGTTTAACTGTATTGATGATCGGTTCTATTATGTTCTCTCTTCTCTTTGC
TCTACTGCCTCCCATTTATGAAAAAAGAATCGTTCAGCACTTTGTCTCACAGCTTGAAGAGGATTTGCTTTACGCTCAGC
AGACGGCTCTCGTCCGCCATACGACGGTTAAGGTGCAGTTTGACTTTGCCGGCTGCCGCTATATCATGAAGCATTCGGAC
GAAGGCTCATCGCCTGAACTGGTCAGGACATACAGCTGCAATATCGCAATCAAAAAGTCGACGCTCAAACCAATTCTTAC
TTTTAATCCAAGCGGTGTTCCGAATCAAGGGGGAAAGATCGTGATCGATACACAGCATGCCTCTTTTATACTGACGATCT
ATTTAGGAAGCGGAAAGATTAATGTTCAAAAAAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB Q8VQ70

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

39.86

98.621

0.393