Detailed information NTG0281818

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL14B4_RS09310
• Genome accession: NZ_CP028160
• Coordinates: 1907824..1908471 (-)
• Length: 215 a.a.
• NCBI ID: WP_033899944.1
• Uniprot ID: A0A2Z3KPW2
• Organism: Lactococcus lactis subsp. lactis strain 14B4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1902824..1913471

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL14B4_RS09285 (LL14B4_09275)	atpF	1902893..1903399 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LL14B4_RS09290 (LL14B4_09280)	atpB	1903414..1904127 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL14B4_RS09295 (LL14B4_09285)	-	1904172..1904387 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL14B4_RS09300 (LL14B4_09290)	-	1904572..1905348 (-)	777	WP_058206315.1	alpha/beta hydrolase	-
LL14B4_RS09305 (LL14B4_09295)	comEC	1905633..1907843 (-)	2211	WP_109991124.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL14B4_RS09310 (LL14B4_09300)	comEA	1907824..1908471 (-)	648	WP_033899944.1	ComEA family DNA-binding protein	Machinery gene
LL14B4_RS09315 (LL14B4_09305)	-	1908531..1909892 (-)	1362	WP_029344292.1	ABC transporter permease	-
LL14B4_RS09320 (LL14B4_09310)	-	1909889..1910821 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL14B4_RS09325 (LL14B4_09315)	-	1910926..1911324 (-)	399	WP_023188528.1	hypothetical protein	-
LL14B4_RS09330 (LL14B4_09320)	-	1911428..1911991 (-)	564	WP_109991125.1	GNAT family N-acetyltransferase	-
LL14B4_RS09335 (LL14B4_09325)	-	1912164..1913342 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23622.79 Da
• Isoelectric Point: 4.8659

>NTDB_id=281818 LL14B4_RS09310 WP_033899944.1 1907824..1908471(-) (comEA) [Lactococcus lactis subsp. lactis strain 14B4]
MDKILEKVKEYWKMIVLVVCGLIAGGVFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LIELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=281818 LL14B4_RS09310 WP_033899944.1 1907824..1908471(-) (comEA) [Lactococcus lactis subsp. lactis strain 14B4]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGGT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGATTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A2Z3KPW2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381