Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9C16_RS00470 Genome accession   NZ_CP032861
Coordinates   74306..74716 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain N1-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 69306..79716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C16_RS00445 (D9C16_00445) - 70412..71302 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  D9C16_RS00450 (D9C16_00450) fumC 71469..72856 (+) 1388 Protein_89 class II fumarate hydratase -
  D9C16_RS22730 - 73075..73287 (+) 213 Protein_90 recombinase family protein -
  D9C16_RS00460 (D9C16_00460) - 73284..73382 (-) 99 WP_031600702.1 hypothetical protein -
  D9C16_RS00465 (D9C16_00465) sigK 73382..74110 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  D9C16_RS00470 (D9C16_00470) nucA/comI 74306..74716 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  D9C16_RS00475 (D9C16_00475) yqeB 74749..75471 (-) 723 WP_014480321.1 hypothetical protein -
  D9C16_RS00480 (D9C16_00480) gnd 75722..76614 (+) 893 Protein_95 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  D9C16_RS00485 (D9C16_00485) yqeD 76633..77259 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  D9C16_RS00490 (D9C16_00490) cwlH 77446..78198 (+) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  D9C16_RS00495 (D9C16_00495) yqeF 78450..79181 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  D9C16_RS00500 (D9C16_00500) - 79487..79627 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=279563 D9C16_RS00470 WP_009967785.1 74306..74716(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain N1-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=279563 D9C16_RS00470 WP_009967785.1 74306..74716(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain N1-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529