Detailed information NTG0278501

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AM480_RS11495
• Genome accession: NZ_CP027611
• Coordinates: 2313033..2314151 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain AR_0101
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2308033..2319151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM480_RS11460 (AM480_11460)	-	2308862..2309710 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AM480_RS11465 (AM480_11465)	-	2309963..2310418 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AM480_RS11475 (AM480_11475)	-	2310571..2311638 (+)	1068	WP_029642282.1	membrane protein	-
AM480_RS11480 (AM480_11480)	-	2311638..2311961 (+)	324	WP_000442585.1	RnfH family protein	-
AM480_RS11485 (AM480_11485)	-	2312016..2312414 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AM480_RS11490 (AM480_11490)	fur	2312526..2312963 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AM480_RS11495 (AM480_11495)	pilU	2313033..2314151 (-)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AM480_RS11500 (AM480_11500)	pilT	2314179..2315216 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AM480_RS11505 (AM480_11505)	-	2315343..2316035 (+)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=278501 AM480_RS11495 WP_000347041.1 2313033..2314151(-) (pilU) [Acinetobacter baumannii strain AR_0101]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=278501 AM480_RS11495 WP_000347041.1 2313033..2314151(-) (pilU) [Acinetobacter baumannii strain AR_0101]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392