Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6I29_RS01235 Genome accession   NZ_CP027365
Coordinates   267020..268729 (-) Length   569 a.a.
NCBI ID   WP_005023073.1    Uniprot ID   A0A062BXA9
Organism   Acinetobacter radioresistens strain DSSKY-A-001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 262020..273729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6I29_RS01205 - 262152..262475 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  C6I29_RS01210 rlmB 262579..263334 (+) 756 WP_005016996.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  C6I29_RS01215 - 263359..264294 (+) 936 WP_005016993.1 DMT family transporter -
  C6I29_RS01220 coaE 264291..264902 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  C6I29_RS01225 pilD 264904..265764 (-) 861 WP_005016989.1 prepilin peptidase Machinery gene
  C6I29_RS01230 pilC 265764..266990 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  C6I29_RS01235 pilB 267020..268729 (-) 1710 WP_005023073.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6I29_RS01240 tpiA 269018..269812 (+) 795 WP_005023075.1 triose-phosphate isomerase -
  C6I29_RS01245 secG 269823..270152 (+) 330 WP_005023077.1 preprotein translocase subunit SecG -
  C6I29_RS01265 rimP 270779..271303 (+) 525 WP_005016981.1 ribosome maturation factor RimP -
  C6I29_RS01270 nusA 271344..272828 (+) 1485 WP_005016980.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63625.89 Da        Isoelectric Point: 5.0245

>NTDB_id=276596 C6I29_RS01235 WP_005023073.1 267020..268729(-) (pilB) [Acinetobacter radioresistens strain DSSKY-A-001]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAIRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNAPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=276596 C6I29_RS01235 WP_005023073.1 267020..268729(-) (pilB) [Acinetobacter radioresistens strain DSSKY-A-001]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAATTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACTCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTATCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATGCGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTCGAGCCTTATGAAAAATATTACC
GAGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTGGCAACACCACCTTTACAGCTGGCCACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAGAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCAACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
TTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGCGCTCC
TGAAACATTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGACTTGGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTAAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062BXA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.383

99.121

0.807
  pilB Acinetobacter baylyi ADP1

78.33

98.946

0.775
  pilB Legionella pneumophila strain ERS1305867

53.676

95.606

0.513
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

92.091

0.476
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50.287

91.916

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.516

100

0.376
  pilF Thermus thermophilus HB27

36.525

99.121

0.362

Multiple sequence alignment