Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   APL_RS04590 Genome accession   NC_009053
Coordinates   1019223..1020617 (-) Length   464 a.a.
NCBI ID   WP_005615186.1    Uniprot ID   A3N0N9
Organism   Actinobacillus pleuropneumoniae serovar 5b str. L20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014223..1025617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APL_RS04560 (APL_0873) lptE 1014837..1015331 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  APL_RS04565 (APL_0874) holA 1015440..1016465 (+) 1026 WP_009874615.1 DNA polymerase III subunit delta -
  APL_RS04570 (APL_0875) yacG 1016542..1016730 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  APL_RS04575 (APL_0876) coaE 1016712..1017350 (-) 639 WP_009874616.1 dephospho-CoA kinase -
  APL_RS04580 (APL_0877) - 1017372..1018010 (-) 639 WP_009874617.1 prepilin peptidase -
  APL_RS04585 (APL_0878) pilC 1018037..1019239 (-) 1203 WP_011848486.1 type II secretion system F family protein Machinery gene
  APL_RS04590 (APL_0879) pilB 1019223..1020617 (-) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  APL_RS04595 (APL_0880) pilA 1020635..1021081 (-) 447 WP_009874618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  APL_RS04600 (APL_0881) radA 1021263..1022642 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  APL_RS04605 (APL_0882) - 1022686..1022919 (-) 234 WP_005597399.1 YgjV family protein -
  APL_RS04610 (APL_0883) folA 1023046..1023534 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  APL_RS04615 (APL_0884) proB 1023621..1024712 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  APL_RS04620 (APL_0885) - 1024725..1025501 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53026.89 Da        Isoelectric Point: 6.5042

>NTDB_id=27616 APL_RS04590 WP_005615186.1 1019223..1020617(-) (pilB) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=27616 APL_RS04590 WP_005615186.1 1019223..1020617(-) (pilB) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCAGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGGCGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N0N9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.499

97.629

0.571
  pilB Haemophilus influenzae 86-028NP

58.057

97.629

0.567
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.414
  pilB Acinetobacter baumannii D1279779

39.059

100

0.412
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Acinetobacter baylyi ADP1

38.525

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment