Detailed information NTG0027598

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: SPH_RS06685
• Genome accession: NC_010380
• Coordinates: 1280994..1281842 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705306.1
• Uniprot ID: Q8DPI7
• Organism: Streptococcus pneumoniae Hungary19A-6
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1275994..1286842

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPH_RS06670 (SPH_1377)	-	1276384..1276743 (-)	360	WP_001237222.1	YbaN family protein	-
SPH_RS06675 (SPH_1378)	topA	1276849..1278936 (-)	2088	WP_000179644.1	type I DNA topoisomerase	-
SPH_RS06680 (SPH_1380)	-	1279103..1280145 (-)	1043	Protein_1289	TIGR00341 family protein	-
SPH_RS06685 (SPH_1382)	dprA/cilB/dalA	1280994..1281842 (-)	849	WP_000705306.1	DNA-processing protein DprA	Machinery gene
SPH_RS06690 (SPH_1383)	-	1281948..1282637 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
SPH_RS06695 (SPH_1384)	-	1282649..1283527 (-)	879	WP_000837609.1	DMT family transporter	-
SPH_RS06700 (SPH_1385)	licA	1283517..1284386 (-)	870	WP_000411204.1	choline kinase LicA	-
SPH_RS06705 (SPH_1386)	-	1284403..1285425 (-)	1023	WP_000609894.1	ribitol-5-phosphate dehydrogenase	-
SPH_RS06710 (SPH_1387)	-	1285430..1286137 (-)	708	WP_000638504.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31062.72 Da
• Isoelectric Point: 5.4047

>NTDB_id=27598 SPH_RS06685 WP_000705306.1 1280994..1281842(-) (dprA/cilB/dalA) [Streptococcus pneumoniae Hungary19A-6]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=27598 SPH_RS06685 WP_000705306.1 1280994..1281842(-) (dprA/cilB/dalA) [Streptococcus pneumoniae Hungary19A-6]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterProScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• PDB: 3UQZ

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	100	100	1
dprA/cilB/dalA	Streptococcus pneumoniae D39	100	100	1
dprA/cilB/dalA	Streptococcus pneumoniae R6	100	100	1
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	100	100	1
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.582	100	0.986
dprA/cilB/dalA	Streptococcus mitis SK321	97.872	100	0.979
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.714	99.291	0.553
dprA	Staphylococcus aureus N315	37.809	100	0.379
dprA	Haemophilus influenzae Rd KW20	38.202	94.681	0.362