Detailed information NTG0270700

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: C3496_RS11090
• Genome accession: NZ_CP026608
• Coordinates: 2167779..2171504 (+)
• Length: 1241 a.a.
• NCBI ID: WP_136444325.1
• Uniprot ID: A0A4S4HWX4
• Organism: Bacillus anthracis strain HDZK-BYSB7
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2162779..2176504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3496_RS11075 (C3496_11575)	-	2162937..2163530 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
C3496_RS11080 (C3496_11580)	lepB	2163587..2164150 (+)	564	WP_071729527.1	signal peptidase I	-
C3496_RS11085 (C3496_11585)	addB	2164267..2167782 (+)	3516	WP_136444326.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
C3496_RS11090 (C3496_11590)	addA	2167779..2171504 (+)	3726	WP_136444325.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
C3496_RS11095 (C3496_11595)	-	2171520..2171807 (+)	288	WP_136444324.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
C3496_RS11100 (C3496_11600)	gerPF	2171844..2172059 (-)	216	WP_001141566.1	spore germination protein GerPF	-
C3496_RS11105 (C3496_11605)	-	2172102..2172488 (-)	387	WP_000902315.1	spore germination protein GerPE	-
C3496_RS11110 (C3496_11610)	gerPD	2172504..2172698 (-)	195	WP_001102340.1	spore germination protein GerPD	-
C3496_RS11115 (C3496_11615)	gerPC	2172705..2173319 (-)	615	WP_001070766.1	spore germination protein GerPC	-
C3496_RS11120 (C3496_11620)	gerPB	2173387..2173593 (-)	207	WP_136444323.1	spore germination protein GerPB	-
C3496_RS11125 (C3496_11625)	gerPA	2173609..2173830 (-)	222	WP_001111188.1	spore germination protein GerPA	-
C3496_RS11130 (C3496_11630)	-	2173927..2174106 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
C3496_RS11135 (C3496_11635)	-	2174354..2175253 (+)	900	WP_001241722.1	fumarylacetoacetate hydrolase family protein	-
C3496_RS11140 (C3496_11640)	-	2175289..2175573 (-)	285	WP_000925336.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142723.06 Da
• Isoelectric Point: 4.8199

>NTDB_id=270700 C3496_RS11090 WP_136444325.1 2167779..2171504(+) (addA) [Bacillus anthracis strain HDZK-BYSB7]
MMENWPKKPEGSQWTDDQWKAVVAKGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGMLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYGEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEILQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=270700 C3496_RS11090 WP_136444325.1 2167779..2171504(+) (addA) [Bacillus anthracis strain HDZK-BYSB7]
ATGATGGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAAGGGGCG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACGGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACGGAGCTCGCAATGCTTCCTGACGGTCCGGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAATGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCAATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCCGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCAATGCAAGATATTCCGCTTGCAGCAGTGCTTCGCTCCCCAATCGTTGGATTAAATGATGAAGAGCTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCGGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAGTGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCTGGAGCGTCTTGTTATTTAGACTGGATTGCCCCTTCCTTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGATGGTAACACTTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAGAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGGAG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAATTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGGGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGTTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

3D structure

• AlphaFold DB: A0A4S4HWX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.747	100	0.537