Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C2U54_RS02075 Genome accession   NZ_CP026167
Coordinates   385977..387410 (+) Length   477 a.a.
NCBI ID   WP_103177192.1    Uniprot ID   -
Organism   Leclercia sp. LSNIH1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380977..392410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U54_RS02045 (C2U54_02045) - 381544..382521 (+) 978 WP_103177187.1 calcium/sodium antiporter -
  C2U54_RS02050 (C2U54_02050) kdsD 382535..383521 (+) 987 WP_103177188.1 arabinose-5-phosphate isomerase KdsD -
  C2U54_RS02055 (C2U54_02055) kdsC 383536..384102 (+) 567 WP_103180991.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C2U54_RS02060 (C2U54_02060) lptC 384099..384674 (+) 576 WP_103177189.1 LPS export ABC transporter periplasmic protein LptC -
  C2U54_RS02065 (C2U54_02065) lptA 384643..385197 (+) 555 WP_103177190.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  C2U54_RS02070 (C2U54_02070) lptB 385204..385929 (+) 726 WP_103177191.1 LPS export ABC transporter ATP-binding protein -
  C2U54_RS02075 (C2U54_02075) rpoN/rpoN1 385977..387410 (+) 1434 WP_103177192.1 RNA polymerase factor sigma-54 Machinery gene
  C2U54_RS02080 (C2U54_02080) hpf 387433..387720 (+) 288 WP_103177193.1 ribosome hibernation promoting factor -
  C2U54_RS02085 (C2U54_02085) ptsN 387803..388294 (+) 492 WP_103177194.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C2U54_RS02090 (C2U54_02090) rapZ 388340..389194 (+) 855 WP_032614487.1 RNase adapter RapZ -
  C2U54_RS02095 (C2U54_02095) npr 389191..389463 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  C2U54_RS02100 (C2U54_02100) mtgA 389460..390185 (-) 726 WP_103177195.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  C2U54_RS02105 (C2U54_02105) elbB 390182..390835 (-) 654 WP_103177196.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54232.08 Da        Isoelectric Point: 4.3230

>NTDB_id=267716 C2U54_RS02075 WP_103177192.1 385977..387410(+) (rpoN/rpoN1) [Leclercia sp. LSNIH1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDEVETQQSQDNETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIELEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIISEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=267716 C2U54_RS02075 WP_103177192.1 385977..387410(+) (rpoN/rpoN1) [Leclercia sp. LSNIH1]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
ACTGTCGACGCTGGAACTGCAGCAGGAGCTCCAGCAAGCGCTGGAAAGTAACCCTCTGCTCGAGCAAACCGATCTTCACG
ATGAGGTAGAAACGCAACAAAGCCAGGATAACGAGACCCTCGACACGGTGGATGCTCTTGAGCAAAAAGAGATGCCTGAC
GAGCTCCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCAGACTACCAGGATGA
CGAGCTGCCGGTCTATCAGGGCGAGACCACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCGTTCT
CCGATACCGATCGCGCCATCGCCACCTCCATTGTGGATGCCGTGGATGAGACCGGCTATCTGACCGTGTCGCTGGACGAT
ATTCTGGAGAGCATAGGCGATGACGAGATTGAGCTTGAAGAGGTCGAAGCGGTCCTGAAACGCGTTCAGCGCTTTGACCC
GATTGGCGTTGCCGCGAGAGATCTCCGTGACTGCCTGCTCATTCAGCTTTCGCAGTTTGTTAAAGAGACCCCGTGGCTGG
AAGAGGCCCGGCTTATCATCAGCGAACATCTGGATCTGCTTGCCAACCACGATTTCCGCTCACTGATGCGCGTCACGCGG
CTGAAAGAGGAGATCCTGAAAGAGGCGGTCAACCTGATCCAGTCGCTGGATCCGCGCCCGGGGCAATCTATCCAGACCAG
CGAGCCGGAGTATGTTATTCCCGATGTGCTGGTGCGCAAACATAACGGTCTGTGGACCGTAGAGCTGAATTCCGACAGCA
TTCCCCGCCTGCAAATCAATCAGCAATACGCCTCAATGTGCACCAGCTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGTAATCTGCAGGAAGCCCGCTGGCTGATCAAAAGCCTTGAGAGCCGCAATGATACACTACTGCGCGTCAGCCGCTGCAT
CGTGGAGCAGCAACAGGCGTTTTTTGAGCAGGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAGTCGACCATTTCTCGCGTGACGACGCAGAAGTATCTGCACAGTCCGCGTGGCATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCGCTGAGTGACAGTAAGTTGACCACCATGCTGTCCGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.525

100

0.491

Multiple sequence alignment