Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C2U32_RS17150 Genome accession   NZ_CP026125
Coordinates   3492214..3493014 (+) Length   266 a.a.
NCBI ID   WP_005135836.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain ABNIH28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3487214..3498014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U32_RS17125 (C2U32_17115) - 3488124..3489422 (+) 1299 WP_086238243.1 glycosyltransferase family 4 protein -
  C2U32_RS17130 (C2U32_17120) - 3489447..3489998 (+) 552 WP_000669563.1 phosphatase PAP2 family protein -
  C2U32_RS17135 (C2U32_17125) iscX 3490098..3490298 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  C2U32_RS17140 (C2U32_17130) ndk 3490412..3490843 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  C2U32_RS17145 (C2U32_17135) rlmN 3490966..3492198 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  C2U32_RS17150 (C2U32_17140) pilF 3492214..3493014 (+) 801 WP_005135836.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C2U32_RS17155 (C2U32_17145) - 3493005..3493796 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  C2U32_RS17160 (C2U32_17150) ispG 3493818..3494933 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C2U32_RS17165 (C2U32_17155) hisS 3494930..3496222 (+) 1293 WP_000095251.1 histidine--tRNA ligase -
  C2U32_RS17170 (C2U32_17160) - 3496251..3496949 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.52 Da        Isoelectric Point: 9.2307

>NTDB_id=267396 C2U32_RS17150 WP_005135836.1 3492214..3493014(+) (pilF) [Acinetobacter baumannii strain ABNIH28]
MSTPKLKIMLCMGVAVALLASGCQTLQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=267396 C2U32_RS17150 WP_005135836.1 3492214..3493014(+) (pilF) [Acinetobacter baumannii strain ABNIH28]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTTGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment