Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CUJ89_RS15495 Genome accession   NZ_CP024902
Coordinates   3195357..3196862 (+) Length   501 a.a.
NCBI ID   WP_114178086.1    Uniprot ID   A0A2Z5MWR8
Organism   Burkholderia pyrrocinia strain mHSR5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3190357..3201862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUJ89_RS15470 (CUJ89_15485) kdsD 3191599..3192582 (+) 984 WP_114178081.1 arabinose 5-phosphate isomerase KdsD -
  CUJ89_RS15475 (CUJ89_15490) - 3192582..3193118 (+) 537 WP_114178082.1 KdsC family phosphatase -
  CUJ89_RS15480 (CUJ89_15495) lptC 3193122..3193736 (+) 615 WP_114178083.1 LPS export ABC transporter periplasmic protein LptC -
  CUJ89_RS15485 (CUJ89_15500) lptA 3193757..3194422 (+) 666 WP_114178084.1 lipopolysaccharide transport periplasmic protein LptA -
  CUJ89_RS15490 (CUJ89_15505) lptB 3194419..3195195 (+) 777 WP_114178085.1 LPS export ABC transporter ATP-binding protein -
  CUJ89_RS15495 (CUJ89_15510) rpoN/rpoN1 3195357..3196862 (+) 1506 WP_114178086.1 RNA polymerase factor sigma-54 Machinery gene
  CUJ89_RS15500 (CUJ89_15515) hpf 3197021..3197380 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CUJ89_RS15505 (CUJ89_15520) - 3197577..3198089 (+) 513 WP_048246965.1 PTS sugar transporter subunit IIA -
  CUJ89_RS15510 (CUJ89_15525) hprK 3198219..3199187 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  CUJ89_RS15515 (CUJ89_15530) rapZ 3199263..3200171 (+) 909 WP_114178088.1 RNase adapter RapZ -
  CUJ89_RS15520 (CUJ89_15535) - 3200198..3200833 (+) 636 WP_114178089.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54829.03 Da        Isoelectric Point: 4.8740

>NTDB_id=256351 CUJ89_RS15495 WP_114178086.1 3195357..3196862(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain mHSR5]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVGSDGSLIAQTPPAQNTEPAA
GNGSSQSGDRAERDEPAGADEYDGYSSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVLQHLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVMP
RLRINSLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=256351 CUJ89_RS15495 WP_114178086.1 3195357..3196862(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain mHSR5]
ATGAAAGCCAGCCTTCAACTTCGCCTGTCGCAACATCTGGCGCTGACACCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTTGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGGGTCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCGGCCGCG
GGGAACGGCAGCAGCCAGTCGGGCGACCGCGCGGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACTCGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGATCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTTGCAGCACCTCGAACTGCTCGCCGCACGCGATTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGACGCGCTACGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATGGTGAAGAAAGTCGGCCAGGGATGGCTCGCGGAACTCAATCCGGAGGTCATGCCG
CGCCTGCGGATCAACAGCCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAAAACATCCAGCAACGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCATGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGATAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTCAATCTGCGTAAGTCTCTTTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment