Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CR152_RS04855 Genome accession   NZ_CP024608
Coordinates   1053479..1054954 (+) Length   491 a.a.
NCBI ID   WP_099873911.1    Uniprot ID   A0A2D2DG05
Organism   Massilia violaceinigra strain B2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048479..1059954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR152_RS04830 (CR152_04830) - 1049931..1050929 (+) 999 WP_099873906.1 SIS domain-containing protein -
  CR152_RS04835 (CR152_04835) - 1050929..1051483 (+) 555 WP_099873907.1 HAD family hydrolase -
  CR152_RS04840 (CR152_04840) lptC 1051505..1052128 (+) 624 WP_099873908.1 LPS export ABC transporter periplasmic protein LptC -
  CR152_RS04845 (CR152_04845) lptA 1052125..1052721 (+) 597 WP_099873909.1 lipopolysaccharide transport periplasmic protein LptA -
  CR152_RS04850 (CR152_04850) lptB 1052721..1053476 (+) 756 WP_099873910.1 LPS export ABC transporter ATP-binding protein -
  CR152_RS04855 (CR152_04855) rpoN/rpoN1 1053479..1054954 (+) 1476 WP_099873911.1 RNA polymerase factor sigma-54 Machinery gene
  CR152_RS04860 (CR152_04860) - 1054977..1056317 (-) 1341 WP_099873912.1 hypothetical protein -
  CR152_RS04865 (CR152_04865) hpf 1056704..1057069 (+) 366 WP_099873913.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CR152_RS04870 (CR152_04870) - 1057168..1058253 (+) 1086 WP_099873914.1 enoyl-CoA hydratase/isomerase family protein -
  CR152_RS04875 (CR152_04875) - 1058340..1058717 (+) 378 WP_099882006.1 BlaI/MecI/CopY family transcriptional regulator -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54450.88 Da        Isoelectric Point: 4.7813

>NTDB_id=254191 CR152_RS04855 WP_099873911.1 1053479..1054954(+) (rpoN/rpoN1) [Massilia violaceinigra strain B2]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLSDNPLLERLDDPLDHSVRLLGDGAISSTSTPTEAAPEPG
AQDAPAPAEGEGFEGGDADSPASSSDNDGEWGDLGRAKTSDDEDSRPQLEASATTLREHLMEQMRVTVLEARDRALVELI
VDAIDDNGYLTESLEEIQERMPPELEIEIEELRTALALLQSFDPIGVGARNASECLALQIRRMPGVALVTRRMALTIVEN
HLTWFAQREFNKLKKALDCDDEDLREAQTVIRQCNPHPGAEFASDVSDYVVPDVIVRKAKNGWSVMLNNDVMPRLRVNSM
YAALLKQGKGEGQMGAQLQEAKWLIKNMRQRFDTILRVAEAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTISRVT
TQKYMMTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTSAEDPKSPLSDSKIADMLGEQGMVIARRTVAKYREAL
KIPPVALRKCL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=254191 CR152_RS04855 WP_099873911.1 1053479..1054954(+) (rpoN/rpoN1) [Massilia violaceinigra strain B2]
ATGAAACAGTCCCTTCAACTACGCACCTCCCAGCACCTCGCCCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCCTGGAACTGCATCAGGAACTGGAGCAGCTGTTAAGCGACAACCCGCTGCTCGAACGGCTCGACGACCCGC
TCGATCATTCGGTGCGCCTGCTGGGCGATGGCGCCATCAGCAGCACCTCGACACCGACCGAAGCGGCGCCCGAGCCGGGA
GCGCAGGATGCGCCGGCGCCGGCCGAAGGGGAAGGCTTCGAAGGCGGCGACGCGGACAGCCCGGCCAGTAGCAGCGACAA
CGATGGCGAATGGGGCGACCTCGGTCGCGCCAAGACATCCGACGACGAGGATTCGCGCCCGCAGCTCGAAGCCTCGGCCA
CCACCTTGCGCGAGCACCTGATGGAGCAGATGCGCGTGACCGTGCTGGAGGCGCGCGACCGCGCGCTGGTCGAACTGATC
GTCGACGCCATCGACGACAACGGCTACCTGACCGAATCGCTGGAAGAAATCCAAGAGCGCATGCCGCCCGAGCTGGAGAT
CGAAATTGAAGAATTGCGCACCGCGCTGGCGCTGCTGCAAAGTTTCGATCCGATTGGCGTGGGCGCGCGCAATGCGTCCG
AATGCCTGGCGCTGCAGATCCGGCGTATGCCGGGCGTGGCGCTGGTGACGCGCCGCATGGCGCTGACGATTGTCGAAAAC
CACCTGACCTGGTTCGCGCAGCGTGAATTCAACAAGCTGAAAAAGGCGCTCGACTGCGACGATGAAGACTTGCGCGAGGC
GCAGACCGTGATCCGCCAGTGCAATCCGCACCCGGGCGCGGAGTTCGCGTCGGACGTGTCGGACTACGTGGTGCCGGACG
TGATCGTGCGCAAGGCGAAAAACGGCTGGTCGGTGATGCTCAATAACGATGTCATGCCGCGCCTGCGCGTGAACAGCATG
TACGCCGCGCTGCTCAAGCAGGGCAAGGGCGAAGGGCAGATGGGCGCGCAGCTGCAGGAAGCGAAGTGGCTGATCAAGAA
CATGCGCCAGCGCTTCGATACCATCTTGCGGGTGGCCGAAGCCATCGTCGAACGCCAGCGCAATTTCTTCTCGCACGGCG
CGGTGGCGATGCGCCCGCTGGTGCTGCGTGAAATCGCCGACACGCTCGGCCTGCACGAGAGTACGATTTCGCGCGTGACC
ACGCAAAAGTACATGATGACGCCGCACGGCATGTTCGAGCTCAAATATTTTTTCGGCAGCCACGTGGCTACCGAAGCGGG
CGGCGAAGCGTCATCGACGGCGATCCGCGCGCTGATCGTGCAGCTGACCAGCGCCGAAGATCCCAAGAGTCCTCTGTCGG
ACAGCAAGATCGCCGACATGCTGGGCGAGCAGGGCATGGTGATCGCGCGCCGCACGGTCGCCAAGTACCGCGAAGCGCTC
AAGATTCCGCCGGTCGCCCTGCGCAAGTGTCTCTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-321)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-489)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.046

100

0.605

Multiple sequence alignment