Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CK627_RS01865 Genome accession   NZ_CP023141
Coordinates   338759..340198 (-) Length   479 a.a.
NCBI ID   WP_017784940.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain KN-Mc-6U21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333759..345198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK627_RS01835 (CK627_01835) - 334034..334774 (+) 741 WP_095591557.1 KDGP aldolase family protein -
  CK627_RS01840 (CK627_01840) - 334879..336783 (+) 1905 WP_095591558.1 transcription antiterminator -
  CK627_RS01845 (CK627_01845) npr 336799..337071 (-) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  CK627_RS01850 (CK627_01850) rapZ 337092..337958 (-) 867 WP_005305946.1 RNase adapter RapZ -
  CK627_RS01855 (CK627_01855) ptsN 338001..338447 (-) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CK627_RS01860 (CK627_01860) hpf 338450..338737 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  CK627_RS01865 (CK627_01865) rpoN/rpoN1 338759..340198 (-) 1440 WP_017784940.1 RNA polymerase factor sigma-54 Machinery gene
  CK627_RS01870 (CK627_01870) lptB 340264..340989 (-) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  CK627_RS01875 (CK627_01875) lptA 340993..341520 (-) 528 WP_095591559.1 lipopolysaccharide transport periplasmic protein LptA -
  CK627_RS01880 (CK627_01880) lptC 341507..342067 (-) 561 WP_005305970.1 LPS export ABC transporter periplasmic protein LptC -
  CK627_RS01885 (CK627_01885) kdsC 342064..342618 (-) 555 WP_017766397.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CK627_RS01890 (CK627_01890) - 342618..343613 (-) 996 WP_005305976.1 KpsF/GutQ family sugar-phosphate isomerase -
  CK627_RS01895 (CK627_01895) mlaF 343821..344621 (+) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53876.83 Da        Isoelectric Point: 4.3584

>NTDB_id=244922 CK627_RS01865 WP_017784940.1 338759..340198(-) (rpoN/rpoN1) [Aeromonas dhakensis strain KN-Mc-6U21]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=244922 CK627_RS01865 WP_017784940.1 338759..340198(-) (rpoN/rpoN1) [Aeromonas dhakensis strain KN-Mc-6U21]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GTGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGATGAATCCGGTTACCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAGCTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCTGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTCTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAAACCTATGCCGCCATGGCCAGACAGGCCCGTACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCAAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486

Multiple sequence alignment