Detailed information NTG0244459

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: CKG21_RS11390
• Genome accession: NZ_CP023046
• Coordinates: 2200169..2200723 (-)
• Length: 184 a.a.
• NCBI ID: WP_006251773.1
• Uniprot ID: -
• Organism: Mannheimia haemolytica strain USDA-ARS-USMARC-187
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2195169..2205723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKG21_RS11375 (CKG21_11375)	sppA	2195252..2197108 (-)	1857	WP_006249718.1	signal peptide peptidase SppA	-
CKG21_RS11380 (CKG21_11380)	-	2197211..2197774 (+)	564	WP_006249720.1	nitroreductase family protein	-
CKG21_RS11385 (CKG21_11385)	-	2197845..2200169 (-)	2325	WP_006249721.1	Tex family protein	-
CKG21_RS11390 (CKG21_11390)	pilF	2200169..2200723 (-)	555	WP_006251773.1	type IV pilus biogenesis/stability protein PilW	Machinery gene
CKG21_RS11395 (CKG21_11395)	-	2200943..2201194 (+)	252	WP_006251772.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CKG21_RS11400 (CKG21_11400)	-	2201178..2201525 (+)	348	WP_006251771.1	helix-turn-helix transcriptional regulator	-
CKG21_RS11405 (CKG21_11405)	-	2201566..2202726 (-)	1161	WP_006253631.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
CKG21_RS11410 (CKG21_11410)	-	2202986..2203882 (+)	897	WP_006251769.1	metal ABC transporter substrate-binding protein	-
CKG21_RS11415 (CKG21_11415)	-	2203895..2204638 (+)	744	WP_006250122.1	metal ABC transporter ATP-binding protein	-
CKG21_RS11420 (CKG21_11420)	-	2204695..2205504 (-)	810	WP_006250123.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 184 a.a.
• Molecular weight: 20976.90 Da
• Isoelectric Point: 7.0805

>NTDB_id=244459 CKG21_RS11390 WP_006251773.1 2200169..2200723(-) (pilF) [Mannheimia haemolytica strain USDA-ARS-USMARC-187]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide

• Length: 555 bp

>NTDB_id=244459 CKG21_RS11390 WP_006251773.1 2200169..2200723(-) (pilF) [Mannheimia haemolytica strain USDA-ARS-USMARC-187]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_17) (95-126)

• Tetratricopeptide repeat (TPR_8) (40-67)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Glaesserella parasuis strain SC1401	63.636	100	0.647
pilF/pilF2	Haemophilus influenzae Rd KW20	47.953	92.935	0.446