Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CJO85_RS14455 Genome accession   NZ_CP022774
Coordinates   3095939..3097438 (+) Length   499 a.a.
NCBI ID   WP_075465400.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain T12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3090939..3102438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJO85_RS14430 (CJO85_14335) - 3092117..3093100 (+) 984 WP_075465395.1 KpsF/GutQ family sugar-phosphate isomerase -
  CJO85_RS14435 (CJO85_14340) - 3093133..3093720 (+) 588 WP_118868631.1 KdsC family phosphatase -
  CJO85_RS14440 (CJO85_14345) lptC 3093722..3094354 (+) 633 WP_075465397.1 LPS export ABC transporter periplasmic protein LptC -
  CJO85_RS14445 (CJO85_14350) lptA 3094423..3095019 (+) 597 WP_013213610.1 lipopolysaccharide transport periplasmic protein LptA -
  CJO85_RS14450 (CJO85_14355) lptB 3095053..3095841 (+) 789 WP_075465399.1 LPS export ABC transporter ATP-binding protein -
  CJO85_RS14455 (CJO85_14360) rpoN/rpoN1 3095939..3097438 (+) 1500 WP_075465400.1 RNA polymerase factor sigma-54 Machinery gene
  CJO85_RS14460 (CJO85_14365) hpf 3097490..3097843 (+) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CJO85_RS14465 (CJO85_14370) ptsN 3098191..3098646 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CJO85_RS14470 (CJO85_14375) hprK 3098717..3099691 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  CJO85_RS14475 (CJO85_14380) - 3099883..3100212 (+) 330 WP_075465401.1 PsiF family protein -
  CJO85_RS14480 (CJO85_14385) rapZ 3100305..3101194 (+) 890 Protein_2827 RNase adapter RapZ -
  CJO85_RS14485 (CJO85_14390) - 3101274..3101924 (+) 651 WP_118883842.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55065.44 Da        Isoelectric Point: 4.8496

>NTDB_id=242252 CJO85_RS14455 WP_075465400.1 3095939..3097438(+) (rpoN/rpoN1) [Ralstonia solanacearum strain T12]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPSANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=242252 CJO85_RS14455 WP_075465400.1 3095939..3097438(+) (rpoN/rpoN1) [Ralstonia solanacearum strain T12]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCTCTGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GTATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGCCTGAAGAAAGCGTTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment